Vilaró Pilar, Sampl Carina, Teichert Gundula, Schlemmer Werner, Hobisch Mathias, Weissl Michael, Panizzolo Luis, Ferreira Fernando, Spirk Stefan
Sede Tacuarembó, Espacio de Ciencia y Tecnología Química, Universidad de la República, CENUR Nores-te. Ruta 5 Km 386, Tacuarembó 45000, Uruguay.
Institute of Bioproducts and Paper Technology, Graz University of Technology, Inffeldgasse 23, 8010 Graz, Austria.
Polymers (Basel). 2020 Dec 18;12(12):3040. doi: 10.3390/polym12123040.
Interactions of biomolecules at interfaces are important for a variety of physiological processes. Among these, interactions of lectins with monosaccharides have been investigated extensively in the past, while polysaccharide-lectin interactions have scarcely been investigated. Here, we explore the adsorption of galactomannans (GM) extracted from on cellulose thin films determined by a combination of multi-parameter surface plasmon resonance spectroscopy (MP-SPR) and atomic force microscopy (AFM). The galactomannan adsorbs spontaneously on the cellulose surfaces forming monolayer type coverage (0.60 ± 0.20 mg·m). The interaction of a lectin, Concavalin A (ConA), with these GM rendered cellulose surfaces using MP-SPR has been investigated and the dissociation constant K (2.1 ± 0.8 × 10 M) was determined in a range from 3.4 to 27.3 nM. The experiments revealed that the galactose side chains as well as the mannose reducing end of the GM are weakly interacting with the active sites of the lectins, whereas these interactions are potentially amplified by hydrophobic effects between the non-ionic GM and the lectins, thereby leading to an irreversible adsorption.
生物分子在界面处的相互作用对多种生理过程都很重要。其中，凝集素与单糖的相互作用在过去已得到广泛研究，而多糖 - 凝集素相互作用则几乎未被研究。在此，我们通过多参数表面等离子体共振光谱（MP - SPR）和原子力显微镜（AFM）相结合的方法，探究了从[具体来源未提及]提取的半乳甘露聚糖（GM）在纤维素薄膜上的吸附情况。半乳甘露聚糖在纤维素表面自发吸附，形成单层覆盖（0.60±0.20 mg·m²）。利用MP - SPR研究了凝集素伴刀豆球蛋白A（ConA）与这些GM修饰的纤维素表面的相互作用，并在3.4至27.3 nM范围内测定了解离常数Kd（2.1±0.8×10⁻⁶ M）。实验表明，GM的半乳糖侧链以及甘露糖还原端与凝集素的活性位点相互作用较弱，而这些相互作用可能因非离子型GM与凝集素之间的疏水作用而增强，从而导致不可逆吸附。
