Institute of Chemistry, University of Graz , Heinrichstraße 28, 8010 Graz, Austria.
Institute for Chemistry and Technology of Materials, Graz University of Technology , Stremayrgasse 9, 8010 Graz, Austria.
Biomacromolecules. 2017 Feb 13;18(2):413-421. doi: 10.1021/acs.biomac.6b01504. Epub 2017 Jan 24.
Polymer-based biomaterials particularly polycaprolactone (PCL) are one of the most promising substrates for tissue engineering. The surface chemistry of these materials plays a major role since it governs protein adsorption, cell adhesion, viability, degradation, and biocompatibility in the first place. This study correlates the interaction of the most abundant serum proteins (albumin, immunoglobulins, fibrinogen) with the surface properties of PCL and its influence on the morphology and metabolic activity of primary human arterial endothelial cells that are seeded on the materials. Prior to that, thin films of PCL are manufactured by spin-coating and characterized in detail. A quartz crystal microbalance with dissipation (QCM-D), a multiparameter surface plasmon resonance spectroscopy instrument (MP-SPR), wettability data, and atomic force microscopy are combined to elucidate the pH-dependent protein adsorption on the PCL substrates. Primary endothelial cells are cultured on the protein modified polymer, and conclusions are drawn on the significant impact of type and form of proteins coatings on cell morphology and metabolic activity.
基于聚合物的生物材料，特别是聚己内酯（PCL），是组织工程中最有前途的基质之一。这些材料的表面化学性质起着主要作用，因为它首先控制着蛋白质的吸附、细胞的黏附、活力、降解和生物相容性。本研究将最丰富的血清蛋白（白蛋白、免疫球蛋白、纤维蛋白原）与 PCL 的表面性质相互作用及其对种植在材料上的原代人动脉内皮细胞形态和代谢活性的影响联系起来。在此之前，通过旋涂法制造 PCL 薄膜，并对其进行详细表征。采用石英晶体微天平（QCM-D）、多参数表面等离子体共振光谱仪（MP-SPR）、润湿性数据和原子力显微镜相结合，阐明了 pH 值对 PCL 基底上蛋白质吸附的影响。将原代内皮细胞培养在蛋白质修饰的聚合物上，并得出关于蛋白质涂层的类型和形式对细胞形态和代谢活性的显著影响的结论。
