Kanpiengjai Apinun, Khanongnuch Chartchai, Lumyong Saisamorn, Kummasook Aksarakorn, Kittibunchakul Suwapat
Division of Biochemistry and Biochemical Technology, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Research Center of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
J Fungi (Basel). 2020 Dec 18;6(4):378. doi: 10.3390/jof6040378.
At present, few yeast species have been evaluated for their beneficial capabilities as probiotics. A45.2, a carotenoid-producing yeast, was able to co-produce cell-associated tannase (CAT), gallic acid and viable cells with antioxidant activity when grown in a tannic acid substrate. The aim of this research study was to identify the potential uses of A45.2 obtained from a co-production system as a potential feed additive for aquaculture. A45.2 and its CAT displayed high tolerance in pH 2.0, pepsin, bile salts and pancreatin. Furthermore, its viable cells were characterized by moderate hydrophobicity, high auto-aggregation and moderate co-aggregation with , ser. Thyphimurium and . These attributes promoted A45.2 as a multifunctional probiotic yeast. In addition, the intact cells possessed antioxidant activities in a 100-150 μg gallic acid equivalent (GAE)/mL culture. Remarkably, the fermentation broth demonstrated higher antioxidant activity of 9.2 ± 1.8, 9.0 ± 0.9, and 9.8 ± 0.7 mg GAE/mL culture after FRAP, DPPH and ABTS assays, respectively. Furthermore, higher antimicrobial activity was observed against , and . Therefore, cultivation of A45.2 with a tannic acid substrate displayed significant potential as an effective multifunctional feed additive.
目前，很少有酵母菌种被评估其作为益生菌的有益能力。A45.2是一种产类胡萝卜素的酵母，当在单宁酸底物中生长时，它能够共同产生细胞相关单宁酶（CAT）、没食子酸和具有抗氧化活性的活细胞。本研究的目的是确定从联产系统中获得的A45.2作为水产养殖潜在饲料添加剂的潜在用途。A45.2及其CAT在pH 2.0、胃蛋白酶、胆汁盐和胰蛋白酶中表现出高耐受性。此外，其活细胞的特征是具有适度的疏水性、高自聚集性以及与鼠伤寒沙门氏菌和肠炎沙门氏菌的适度共聚集性。这些特性使A45.2成为一种多功能益生菌酵母。此外，完整细胞在100 - 150μg没食子酸当量（GAE）/mL培养物中具有抗氧化活性。值得注意的是，发酵液在FRAP、DPPH和ABTS测定后分别显示出更高的抗氧化活性，分别为9.2±1.8、9.0±0.9和9.8±0.7mg GAE/mL培养物。此外，观察到对大肠杆菌、金黄色葡萄球菌和白色念珠菌具有更高的抗菌活性。因此，用单宁酸底物培养A45.2作为一种有效的多功能饲料添加剂具有显著潜力。
