College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Hunan, China.
Mediators Inflamm. 2020 Nov 28;2020:1240152. doi: 10.1155/2020/1240152. eCollection 2020.
Previous study showed that low protein diet-fed pigs are characterized by lower histidine concentration in the serum and muscle, suggesting that histidine may involve in protein-restricted response. Thus, the current study mainly investigated the effects of dietary histidine on growth performance, blood biochemical parameters and amino acids, intestinal morphology, and microbiota communities in low protein diet-challenged-piglets. The results showed that protein restriction inhibited growth performance, blood biochemical parameters and amino acids, and gut microbiota but had little effect on intestinal morphology. Dietary supplementation with histidine markedly enhanced serum histidine level and restored tryptophan concentration in low protein diet-fed piglets, while growth performance and intestinal morphology were not markedly altered in histidine-treated piglets. In addition, histidine exposure failed to affect bacterial diversity (observed species, Shannon, Simpson, Chao1, ACE, and phylogenetic diversity), but histidine-treated piglets exhibited higher abundances of and compared with the control and protein-restricted piglets. In conclusion, dietary histidine in low protein diet enhanced histidine concentration and affected gut microbiota ( and ) but failed to improve growth performance and intestinal morphology.
先前的研究表明，低蛋白饮食喂养的猪的血清和肌肉中组氨酸浓度较低，这表明组氨酸可能参与了蛋白质限制反应。因此，本研究主要研究了日粮组氨酸对低蛋白饮食挑战仔猪生长性能、血液生化参数和氨基酸、肠道形态和微生物群落的影响。结果表明，蛋白质限制抑制了生长性能、血液生化参数和氨基酸，以及肠道微生物群，但对肠道形态影响不大。日粮补充组氨酸显著提高了血清组氨酸水平，并恢复了低蛋白饮食喂养仔猪的色氨酸浓度，而组氨酸处理的仔猪的生长性能和肠道形态没有明显改变。此外，组氨酸暴露并未影响细菌多样性（观察到的物种、香农、辛普森、蔡氏、ACE 和系统发育多样性），但与对照组和蛋白质限制组相比，组氨酸处理组的 和 的丰度更高。总之，低蛋白饮食中的组氨酸增强了组氨酸浓度并影响了肠道微生物群（和），但未能改善生长性能和肠道形态。
