水果通过调节肠道菌群和抑制 NF-B 通路对葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎的抗炎作用。

Anti-Inflammatory Effects of Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-B Pathway.

机构信息

Research Center of Chinese Herbal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.

School of Pharmaceutical Science (Mathematical Engineering Academy of Chinese Medicine), Guangzhou University of Chinese Medicine, Guangzhou 510006, China.

出版信息

Biomed Res Int. 2020 Dec 5;2020:8893621. doi: 10.1155/2020/8893621. eCollection 2020.

MATERIALS AND METHODS

The chemical compositions of EFH were identified using LC-ESI-MS. The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage. The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators. The gut microbiota was characterized by 16S rRNA gene sequencing and analysis.

RESULTS

LC-ESI-MS analysis showed that EFH was rich in alkaloids and flavones. The results indicated that EFH significantly improved the DAI score, relieved colon shortening, and repaired pathological colonic variations in colitis. In addition, proteins in the NF-B pathway were significantly inhibited by EFH. Furthermore, EFH recovered the diversity and balance of the gut microbiota.

CONCLUSIONS

EFH has protective effects against DSS-induced colitis by keeping the balance of the gut microbiota and suppressing the NF-B pathway.

材料和方法

采用 LC-ESI-MS 鉴定 EFH 的化学成分。将 3% DSS 诱导的 UC 小鼠通过每日灌胃给予 EFH（200、400 和 800mg/kg）、柳氮磺胺吡啶（SASP，200mg/kg）和硫唑嘌呤（AZA，13mg/kg）治疗 10 天。通过疾病活动指数（DAI）、结肠长度、组织学评分和炎症介质水平评估结肠炎症。通过 16S rRNA 基因测序和分析来描述肠道微生物群。