中国大瑶山富含可可碱的野生茶树（Camellia gymnogyna Chang）中可可碱合成酶的纯化与表征。
Purification and characterization of theobromine synthase in a Theobromine-Enriched wild tea plant (Camellia gymnogyna Chang) from Dayao Mountain, China.
机构信息
Department of Tea Science, College of Horticulture, South China Agricultural University, Guangzhou 510642, China; Department of Tea Science, School of Agricultural Sciences, Jiangxi Agricultural University, Nanchang 330045, China.
Department of Tea Science, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
出版信息
Food Chem. 2020 May 1;311:125875. doi: 10.1016/j.foodchem.2019.125875. Epub 2019 Nov 12.
Camellia gymnogyna Chang (CgC), a wild tea plant, was discovered on Dayao Mountain, China. However, research regarding this tea plant is limited. Our study found that CgC contains theobromine, caffeine, and theacrine, among which theobromine content was the highest (14.37-39.72 mg/g). In addition, theobromine synthase (TS) was partially purified from CgC leaves, up to 35.87-fold, with consecutive chromatography, and its molecular weight was found to be approximately 62 kDa. The optimum reaction time, pH, and temperature for theobromine synthase from 7-methylxanthine was found to be 6 h, 4, and 45 °C, respectively. TS expression at both mRNA and protein stages was higher in the first than in the fourth leaf (P < 0.05). Subcellular localization of TS indicated that it was localized in the nucleus. These results indicate that CgC can be of scientific value and could lead to efficient utilization of this rare wild tea germplasm.
野茶树（Camellia gymnogyna Chang，CgC）于中国大尧山被发现，其为一种野生茶树，但目前对该茶树的研究有限。我们的研究发现，CgC 含有可可碱、咖啡因和可可因，其中可可碱含量最高（14.37-39.72mg/g）。此外，我们从 CgC 叶片中部分纯化出可可碱合成酶（TS），经过连续层析，其纯度提高了 35.87 倍，分子量约为 62kDa。7-甲基黄嘌呤的可可碱合成酶的最佳反应时间、pH 值和温度分别为 6 小时、4 和 45°C。在第一片和第四片叶子中，TS 在 mRNA 和蛋白质水平上的表达均更高（P<0.05）。TS 的亚细胞定位表明其定位于细胞核。这些结果表明，CgC 具有科学价值，并可能导致对这种稀有野生茶树种质资源的有效利用。
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