SpheriCal-ESI：一种基于树枝状大分子的九点校准溶液，分子量范围为 273 至 1716，用于电喷雾电离质谱肽分析。

SpheriCal -ESI: A dendrimer-based nine-point calibration solution ranging from m/z 273 to 1716 for electrospray ionization mass spectrometry peptide analysis.

机构信息

School of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Chemistry, Division of Applied Physical Chemistry, Analytical Chemistry, KTH Royal Institute of Technology, Stockholm, Sweden.

School of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Fibre and Polymer Technology, Division of Coating Technology, KTH Royal Institute of Technology, Stockholm, Sweden.

出版信息

Rapid Commun Mass Spectrom. 2021 Mar 15;35(5):e9035. doi: 10.1002/rcm.9035.

DOI:10.1002/rcm.9035

PMID:33354835

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7900979/

Abstract

RATIONALE

A calibration solution for mass spectrometry needs to cover the range of interest with intense and sufficiently narrowly spaced peaks. Limited options fulfilling this may lead to compromises between performance and ease of use. SpheriCal -ESI was designed to combine high calibration performance for electrospray ionization (ESI) mass spectrometric analysis of peptides in positive mode with quick and easy use.

METHODS

The developed calibration solution was tested using three mass spectrometers: two ion traps and one tandem quadrupole. The m/z errors of SpheriCal -ESI itself and of a tryptic digest of cytochrome C were measured after calibration. The results were compared with those achieved with ESI Tuning Mix. The memory effects of the dendrimers, and contamination from Na in the calibration solution, were evaluated.

RESULTS

SpheriCal -ESI showed good shelf life as powder and was quickly reconstituted for use. Achieving intense and stable signals was straightforward. The accuracies and precisions were as expected for the instruments. SpheriCal -ESI was more precise and at least as accurate as ESI Tuning Mix. The memory effects and Na contamination were found to be negligible in typical peptide solvents. In addition, the dendrimers showed predictable dissociations with product ions common to collision-induced dissociation in both ion trap and tandem quadrupole mass spectrometers.

CONCLUSIONS

SpheriCal -ESI provided easily accessible calibration by showing intense signals at low infusion rates and at source settings equal or similar to those used in peptide analysis. Nine calibration points in the range of interest gave precise and accurate results. Memory effects and contamination were negligible even without rinsing.

摘要

原理

质谱分析用的校准溶液需要在感兴趣的范围内具有高强度和足够窄的峰间距。为了满足这个要求，可供选择的方案可能在性能和易用性之间存在折衷。SpheriCal-ESI 的设计目的是结合电喷雾电离（ESI）质谱分析正模式下肽的高校准性能，以及快速和简单的使用。

方法

使用三台质谱仪（两台离子阱和一台串联四极杆）测试了所开发的校准溶液。用校准后的 SpheriCal-ESI 和细胞色素 C 的胰蛋白酶消化物测量 m/z 误差。将结果与 ESI Tuning Mix 的结果进行了比较。评估了树枝状大分子的记忆效应和校准溶液中 Na 的污染。

结果

SpheriCal-ESI 作为粉末具有良好的保质期，且可以迅速复溶使用。实现强烈而稳定的信号非常简单。仪器的准确度和精密度都符合预期。SpheriCal-ESI 比 ESI Tuning Mix 更精确，至少同样准确。在典型的肽溶剂中，发现记忆效应和 Na 污染可以忽略不计。此外，树枝状大分子在离子阱和串联四极杆质谱仪中都表现出与碰撞诱导解离中常见的产物离子可预测的解离。