Zhejiang Osmum Biological Co., Ltd, Huzhou, China.
Zhejiang Osmum Group Deqing Biotechnology Co., Ltd, Huzhou, China.
J Cosmet Dermatol. 2021 Aug;20(8):2531-2537. doi: 10.1111/jocd.13899. Epub 2021 Jan 8.
Incidence of skin pigmentation disorders has been on the rise globally. This calls for safer and more effective topical skin lightening and freckle-removing products. In this study, we hypothesized that Soluble Pearl Extract (SPE) may possess endothelin antagonizing compounds with good skin whitening effects.
(a) To determine the effect and mechanisms of SPE on ET-1-treated B16 melanoma cells. (b) To explore the cytotoxic effects of SPE on B16 melanoma cells.
CCK-8 assay was performed to determine how SPE and ET-1 affect the proliferation rate of B16 melanoma cells, the NaOH lysis assay was conducted to quantify the content of melanin while the tyrosinase activity was determined by DOPA oxidation test. The mRNA and protein expression levels of TYR and TRP-1 were determined by qRT-PCR assay and Western blot assay, respectively.
We found that SPE at 0.1 and 1 μg/mL concentrations has no effect on the proliferation of the cells and 10 nmol/L ET-1 promoted B16 melanoma cells proliferation. Notably, B16 melanoma cells treated with 10 nmol/L ET-1 exhibited significantly higher melanin synthesis, tyrosinase activity, TYR, and TRP-1 mRNA expression levels compared with untreated cells. Of note, the effects of 10 nmol/L ET-1 treatment were abolished with SPE in a dose-dependent manner.
SPE inhibits endothelin thereby safely and effectively lightening lightens the skin by antagonizing endothelin. Moreover, SPE is safe and effective.
全球范围内皮肤色素沉着障碍的发病率一直在上升。这就需要更安全、更有效的局部皮肤美白和祛斑产品。在这项研究中，我们假设珍珠提取物（SPE）可能含有具有良好皮肤美白效果的内皮素拮抗化合物。
（a）确定 SPE 对 ET-1 处理的 B16 黑素瘤细胞的作用和机制。（b）探讨 SPE 对 B16 黑素瘤细胞的细胞毒性作用。
CCK-8 法测定 SPE 和 ET-1 对 B16 黑素瘤细胞增殖率的影响，NaOH 裂解法测定黑素含量，多巴氧化试验测定酪氨酸酶活性。通过 qRT-PCR 法和 Western blot 法分别测定 TYR 和 TRP-1 的 mRNA 和蛋白表达水平。
我们发现 0.1 和 1μg/ml 浓度的 SPE 对细胞的增殖没有影响，而 10nmol/L ET-1 促进了 B16 黑素瘤细胞的增殖。值得注意的是，与未处理的细胞相比，用 10nmol/L ET-1 处理的 B16 黑素瘤细胞的黑色素合成、酪氨酸酶活性、TYR 和 TRP-1 mRNA 表达水平显著升高。值得注意的是，10nmol/L ET-1 处理的作用被 SPE 以剂量依赖性方式消除。
SPE 通过拮抗内皮素抑制内皮素，从而安全有效地提亮皮肤。此外，SPE 安全有效。
