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从分离的蛋白质消化后释放的新型生物活性肽序列。

Novel bioactive peptides sequences released by digestion of proteins isolated from .

机构信息

Chemistry Investigation Center, Universidad Autónoma del Estado de Hidalgo, Mineral de la Reforma, Hidalgo, Mexico.

Biotechnology Department, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico.

出版信息

Nat Prod Res. 2022 Jul;36(13):3485-3488. doi: 10.1080/14786419.2020.1862837. Epub 2020 Dec 28.

DOI:10.1080/14786419.2020.1862837
PMID:33356582
Abstract

Amaranth is a protein-rich seed, highly consumed in Mexico and reported as a potential source of bio-peptides with physiological function. Therefore, the objective of this study was to evaluate and identify bioactive peptide sequences from amaranth protein fractions released by pepsin and pancreatin digestion. Multifunctional bioactivities of peptides were measured through antihypertensive, antithrombotic and antioxidant assays. Peptide sequences were identified by analysis with a Nano-LC-ESI-MS/MS. With these results, it was demonstrated that the enzymatic digestion mechanism allows the release of multifunctional bioactive peptide fractions from amaranth proteins.

摘要

苋菜籽是一种富含蛋白质的种子,在墨西哥被大量食用,并被报道为具有生理功能的生物肽的潜在来源。因此,本研究的目的是评估和鉴定胃蛋白酶和胰蛋白酶消化释放的苋菜籽蛋白级分中的生物活性肽序列。通过抗高血压、抗血栓和抗氧化测定来测量肽的多功能生物活性。通过 Nano-LC-ESI-MS/MS 分析鉴定肽序列。有了这些结果,证明了酶解机制允许从苋菜籽蛋白中释放具有多种功能的生物活性肽级分。

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