Suárez S E, Quiroga A, Sabbione A C, Rodríguez M, Nardo A E, Jardin J, Scilingo A, Tironi V, Speroni F, Añón M C
Laboratorio de Investigación, Desarrollo e Innovación de Proteínas Alimentarias (LIDiPA), Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA, CONICET-CIC-UNLP), Street 47 and 116, La Plata, Buenos Aires, Argentina.
Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Buenos Aires, Argentina.
Plant Foods Hum Nutr. 2024 Dec 30;80(1):27. doi: 10.1007/s11130-024-01243-1.
Beverages formulated from alternative proteins, such as amaranth, are gaining attraction due to changes in human dietary patterns and environmental concerns like resource use and biodiversity loss. This study focuses on assessing the bioactive peptide release from an amaranth protein beverage. This beverage was subjected to a static simulated gastrointestinal digestion (SGD) protocol to evaluate its bioaccessibility and functional potential. The digests were analyzed for in vitro antihypertensive, antioxidant, and antithrombotic activities. Additionally, digested peptide sequences were identified via LC-MS/MS. The results showed that SGD significantly enhanced the release of bioactive peptides, leading to increased ACE inhibition, antioxidant capacity against ABTS + and AAPH-induced radicals, and antithrombotic effects in clotting assays. LC-MS/MS analysis identified 31 peptides in the digests, ranging from 9 to 21 amino acids, associated with various bioactivities. These findings highlight amaranth proteins potential as a source of functional peptides with health-promoting properties. Further research must be done to isolate and characterize specific peptides for potential therapeutic applications.
由苋菜等替代蛋白质制成的饮料，因人类饮食模式的变化以及对资源利用和生物多样性丧失等环境问题的关注而越来越受到关注。本研究重点评估苋菜蛋白饮料中生物活性肽的释放情况。该饮料采用静态模拟胃肠消化（SGD）方案进行处理，以评估其生物可及性和功能潜力。对消化产物进行体外抗高血压、抗氧化和抗血栓形成活性分析。此外，通过LC-MS/MS鉴定消化后的肽序列。结果表明，SGD显著增强了生物活性肽的释放，导致ACE抑制作用增强、对ABTS+和AAPH诱导的自由基的抗氧化能力增强以及凝血试验中的抗血栓形成作用增强。LC-MS/MS分析在消化产物中鉴定出31种肽，氨基酸数量从9到21个不等，与各种生物活性相关。这些发现突出了苋菜蛋白作为具有健康促进特性的功能肽来源的潜力。必须开展进一步研究，以分离和表征特定肽用于潜在的治疗应用。
