Zhangjiakou Academy of Agricultural Sciences, Zhangjiakou, Hebei, China.
Hengshui University, Hengshui, Hebei, China.
PeerJ. 2022 Jun 28;10:e13669. doi: 10.7717/peerj.13669. eCollection 2022.
The growth and development of naked oat ( L.) seedlings, a grain recognized as nutritious and healthy, is limited by drought. Melatonin plays a positive role in plants under drought stress. However, its function is unclear in naked oats. This study demonstrated that melatonin enhances drought stress tolerance in oat seedlings. Melatonin application alleviated the declining growth parameters of two naked oat varieties, Huazao No.2 (H2) and Jizhangyou No.15 (J15), under drought stress by increasing the chlorophyll content and photosynthetic rate of leaves. Melatonin pretreatment induced differential gene expression in H2 and J15 under drought stress. Subsequently, the differential gene expression responses to melatonin in the two varieties were further analyzed. The key drought response transcription factors and the regulatory effect of melatonin on drought-related transcription factors were assessed, focusing on genes encoding proteins in the ABA signal transduction pathway, including , , , , and . Taken together, this study provides new insights into the effect and underlying mechanism of melatonin in alleviating drought stress in naked oat seedlings.
裸燕麦（L.）幼苗的生长和发育受到干旱的限制，裸燕麦是一种被认为营养健康的谷物。褪黑素在植物应对干旱胁迫中发挥着积极作用，但在裸燕麦中的功能尚不清楚。本研究表明，褪黑素增强了燕麦幼苗对干旱胁迫的耐受性。褪黑素的应用通过增加叶片的叶绿素含量和光合速率，缓解了华早 2 号（H2）和冀张油 15 号（J15）两个裸燕麦品种在干旱胁迫下生长参数下降的情况。褪黑素预处理诱导了 H2 和 J15 在干旱胁迫下的差异基因表达。随后，进一步分析了两种品种对褪黑素的差异基因表达反应。评估了关键干旱响应转录因子以及褪黑素对干旱相关转录因子的调控作用，重点关注 ABA 信号转导途径中编码蛋白质的基因，包括 、 、 、 和 。综上所述，本研究为褪黑素缓解裸燕麦幼苗干旱胁迫的作用和机制提供了新的见解。
