• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

对人类 tRNA 池的操纵揭示了在细胞增殖或细胞周期停滞中起作用的不同 tRNA 集。

Manipulation of the human tRNA pool reveals distinct tRNA sets that act in cellular proliferation or cell cycle arrest.

机构信息

Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.

Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.

出版信息

Elife. 2020 Dec 24;9:e58461. doi: 10.7554/eLife.58461.

DOI:10.7554/eLife.58461
PMID:33357381
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7781600/
Abstract

Different subsets of the tRNA pool in human cells are expressed in different cellular conditions. The 'proliferation-tRNAs' are induced upon normal and cancerous cell division, while the 'differentiation-tRNAs' are active in non-dividing, differentiated cells. Here we examine the essentiality of the various tRNAs upon cellular growth and arrest. We established a CRISPR-based editing procedure with sgRNAs that each target a tRNA family. We measured tRNA essentiality for cellular growth and found that most proliferation-tRNAs are essential compared to differentiation- tRNAs in rapidly growing cell lines. Yet in more slowly dividing lines, the differentiation-tRNAs were more essential. In addition, we measured the essentiality of each tRNA family upon response to cell cycle arresting signals. Here we detected a more complex behavior with both proliferation-tRNAs and differentiation tRNAs showing various levels of essentiality. These results provide the so-far most comprehensive functional characterization of human tRNAs with intricate roles in various cellular states.

摘要

不同亚群的 tRNA 在人类细胞中表达于不同的细胞状态。“增殖-tRNA”在正常和癌变细胞分裂时被诱导，而“分化-tRNA”在非分裂、分化的细胞中活跃。在此，我们研究了各种 tRNA 在细胞生长和停滞时的必要性。我们建立了一种基于 CRISPR 的编辑程序，使用 sgRNA 靶向每个 tRNA 家族。我们测量了 tRNA 对细胞生长的必要性，发现与分化-tRNA 相比，大多数增殖-tRNA 在快速生长的细胞系中是必需的。然而，在分裂较慢的细胞系中，分化-tRNA 更为必需。此外，我们测量了每个 tRNA 家族在响应细胞周期阻滞信号时的必要性。在这里，我们检测到一种更复杂的行为，增殖-tRNA 和分化-tRNA 表现出不同程度的必需性。这些结果提供了迄今为止最全面的人类 tRNA 功能特征，揭示了其在各种细胞状态下复杂的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/c113d502257d/elife-58461-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/1a4ef13b08e2/elife-58461-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/20914ff81590/elife-58461-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/361826f4ba22/elife-58461-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/fe1294f8e362/elife-58461-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/2059fa79d6f4/elife-58461-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/d279d32641e6/elife-58461-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/f239d27c1bb7/elife-58461-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/8edea5a7401f/elife-58461-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/983f900464a4/elife-58461-fig4-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/a1698879341a/elife-58461-fig4-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/c13921f5a79a/elife-58461-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/c113d502257d/elife-58461-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/1a4ef13b08e2/elife-58461-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/20914ff81590/elife-58461-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/361826f4ba22/elife-58461-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/fe1294f8e362/elife-58461-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/2059fa79d6f4/elife-58461-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/d279d32641e6/elife-58461-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/f239d27c1bb7/elife-58461-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/8edea5a7401f/elife-58461-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/983f900464a4/elife-58461-fig4-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/a1698879341a/elife-58461-fig4-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/c13921f5a79a/elife-58461-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c2a/7781600/c113d502257d/elife-58461-fig5-figsupp1.jpg

相似文献

1
Manipulation of the human tRNA pool reveals distinct tRNA sets that act in cellular proliferation or cell cycle arrest.对人类 tRNA 池的操纵揭示了在细胞增殖或细胞周期停滞中起作用的不同 tRNA 集。
Elife. 2020 Dec 24;9:e58461. doi: 10.7554/eLife.58461.
2
Controlling translation via modulation of tRNA levels.通过调节tRNA水平来控制翻译。
Wiley Interdiscip Rev RNA. 2015 Jul-Aug;6(4):453-70. doi: 10.1002/wrna.1287. Epub 2015 Apr 28.
3
tRNA biogenesis and specific aminoacyl-tRNA synthetases regulate senescence stability under the control of mTOR.tRNA 生物发生和特定的氨酰-tRNA 合成酶在 mTOR 的控制下调节衰老稳定性。
PLoS Genet. 2021 Dec 20;17(12):e1009953. doi: 10.1371/journal.pgen.1009953. eCollection 2021 Dec.
4
Post-transcriptional regulation of the steady-state levels of mitochondrial tRNAs in HeLa cells.HeLa细胞中线粒体tRNA稳态水平的转录后调控。
J Biol Chem. 1993 May 15;268(14):10228-37.
5
Dynamic changes in tRNA modifications and abundance during T cell activation.T 细胞活化过程中 tRNA 修饰和丰度的动态变化。
Proc Natl Acad Sci U S A. 2021 Oct 19;118(42). doi: 10.1073/pnas.2106556118.
6
Developmental roles of Drosophila tRNA processing endonuclease RNase ZL as revealed with a conditional rescue system.果蝇 tRNA 加工内切酶 RNase ZL 的发育作用，通过条件性拯救系统揭示。
Dev Biol. 2013 Sep 15;381(2):324-40. doi: 10.1016/j.ydbio.2013.07.005. Epub 2013 Jul 15.
7
Genomic innovation of ATD alleviates mistranslation associated with multicellularity in Animalia.动物界中 ATD 的基因组创新缓解了与多细胞性相关的翻译错误。
Elife. 2020 May 28;9:e58118. doi: 10.7554/eLife.58118.
8
An La-related protein controls cell cycle arrest by nuclear retrograde transport of tRNAs during diapause formation in Artemia.一种与La相关的蛋白质通过卤虫滞育形成过程中tRNA的核逆行转运来控制细胞周期停滞。
BMC Biol. 2016 Mar 3;14:16. doi: 10.1186/s12915-016-0239-4.
9
Genome-wide analysis of N1-methyl-adenosine modification in human tRNAs.人类 tRNA 中 N1-甲基腺苷修饰的全基因组分析。
RNA. 2010 Jul;16(7):1317-27. doi: 10.1261/rna.2057810. Epub 2010 May 19.
10
Harnessing tRNA for Processing Ability and Promoter Activity.利用 tRNA 的加工能力和启动子活性。
Methods Mol Biol. 2021;2162:89-114. doi: 10.1007/978-1-0716-0687-2_7.

引用本文的文献

1
Design and validation of cell-based potency assays for frataxin supplementation treatments.用于补充法布莱病蛋白治疗的基于细胞的效价测定的设计与验证
Mol Ther Methods Clin Dev. 2024 Sep 27;32(4):101347. doi: 10.1016/j.omtm.2024.101347. eCollection 2024 Dec 12.
2
SARS-CoV-2 Displays a Suboptimal Codon Usage Bias for Efficient Translation in Human Cells Diverted by Hijacking the tRNA Epitranscriptome.SARS-CoV-2 在人类细胞中表现出低效的翻译密码子使用偏性，这种偏性是通过劫持 tRNA 表观转录组实现的。
Int J Mol Sci. 2024 Oct 29;25(21):11614. doi: 10.3390/ijms252111614.
3
tRNA and tsRNA: From Heterogeneity to Multifaceted Regulators.

本文引用的文献

1
Engineered materials for in vivo delivery of genome-editing machinery.用于体内递送基因组编辑机制的工程材料。
Nat Rev Mater. 2019 Nov;4:726-737. doi: 10.1038/s41578-019-0145-9. Epub 2019 Oct 4.
2
Control of translation elongation in health and disease.在健康和疾病中控制翻译延伸。
Dis Model Mech. 2020 Mar 26;13(3):dmm043208. doi: 10.1242/dmm.043208.
3
Proteomic and interactomic insights into the molecular basis of cell functional diversity.蛋白质组学和相互作用组学揭示细胞功能多样性的分子基础。
tRNA 和 tsRNA：从异质性到多功能调控因子。
Biomolecules. 2024 Oct 21;14(10):1340. doi: 10.3390/biom14101340.
4
Tyrosine transfer RNA levels and modifications during blood-feeding and vitellogenesis in the mosquito, Aedes aegypti.埃及伊蚊在吸血和卵黄发生过程中的酪氨酸转运RNA水平及修饰
Insect Mol Biol. 2025 Feb;34(1):65-80. doi: 10.1111/imb.12950. Epub 2024 Aug 6.
5
Orb2 enables rare-codon-enriched mRNA expression during Drosophila neuron differentiation.Orb2 在果蝇神经元分化过程中促进稀有密码子富集 mRNA 的表达。
Nat Commun. 2024 Jun 20;15(1):5270. doi: 10.1038/s41467-024-48344-8.
6
Metabolism-dependent secondary effect of anti-MAPK cancer therapy on DNA repair.抗丝裂原活化蛋白激酶（MAPK）癌症治疗对DNA修复的代谢依赖性继发效应。
NAR Cancer. 2024 Apr 30;6(2):zcae019. doi: 10.1093/narcan/zcae019. eCollection 2024 Jun.
7
The choreography of chromatin in RNA polymerase III regulation.RNA 聚合酶 III 调控中染色质的构象变化。
Biochem Soc Trans. 2024 Jun 26;52(3):1173-1189. doi: 10.1042/BST20230770.
8
Advances in methods for tRNA sequencing and quantification.tRNA 测序和定量方法的进展。
Trends Genet. 2024 Mar;40(3):276-290. doi: 10.1016/j.tig.2023.11.001. Epub 2023 Dec 19.
9
Tyrosine transfer RNA levels and modifications during blood-feeding and vitellogenesis in the mosquito, .蚊子吸血和卵黄发生过程中的酪氨酸转运RNA水平及修饰
bioRxiv. 2023 Nov 29:2023.11.29.569187. doi: 10.1101/2023.11.29.569187.
10
Locus-specific proteome decoding reveals Fpt1 as a chromatin-associated negative regulator of RNA polymerase III assembly.基因座特异性蛋白质组解码揭示Fpt1是RNA聚合酶III组装的染色质相关负调控因子。
Mol Cell. 2023 Dec 7;83(23):4205-4221.e9. doi: 10.1016/j.molcel.2023.10.037. Epub 2023 Nov 22.
Nat Rev Mol Cell Biol. 2020 Jun;21(6):327-340. doi: 10.1038/s41580-020-0231-2. Epub 2020 Mar 31.
4
Translational efficiency across healthy and tumor tissues is proliferation-related.健康组织和肿瘤组织中的翻译效率与增殖有关。
Mol Syst Biol. 2020 Mar;16(3):e9275. doi: 10.15252/msb.20199275.
5
Identification and dynamic quantification of regulatory elements using total RNA.使用总 RNA 鉴定和动态定量调控元件。
Genome Res. 2019 Nov;29(11):1836-1846. doi: 10.1101/gr.253492.119. Epub 2019 Oct 24.
6
Systematic Detection of Amino Acid Substitutions in Proteomes Reveals Mechanistic Basis of Ribosome Errors and Selection for Translation Fidelity.系统检测蛋白质组中的氨基酸替换揭示了核糖体错误的机制基础和翻译保真度的选择。
Mol Cell. 2019 Aug 8;75(3):427-441.e5. doi: 10.1016/j.molcel.2019.06.041. Epub 2019 Jul 25.
7
Transcriptome signature of cellular senescence.细胞衰老的转录组特征。
Nucleic Acids Res. 2019 Aug 22;47(14):7294-7305. doi: 10.1093/nar/gkz555.
8
tRNA Deregulation and Its Consequences in Cancer.tRNA 失调及其在癌症中的后果。
Trends Mol Med. 2019 Oct;25(10):853-865. doi: 10.1016/j.molmed.2019.05.011. Epub 2019 Jun 24.
9
The multiple mechanisms that regulate p53 activity and cell fate.调控 p53 活性和细胞命运的多种机制。
Nat Rev Mol Cell Biol. 2019 Apr;20(4):199-210. doi: 10.1038/s41580-019-0110-x.
10
Global analysis of tRNA and translation factor expression reveals a dynamic landscape of translational regulation in human cancers.tRNA和翻译因子表达的全局分析揭示了人类癌症中翻译调控的动态图景。
Commun Biol. 2018 Dec 21;1:234. doi: 10.1038/s42003-018-0239-8. eCollection 2018.