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番木瓜花叶病毒RNA衣壳蛋白基因及3'非编码区的核苷酸序列

Nucleotide sequence of the capsid protein gene and 3' non-coding region of papaya mosaic virus RNA.

作者信息

Abouhaidar M G

机构信息

Department of Botany, University of Toronto, Ontario, Canada.

出版信息

J Gen Virol. 1988 Jan;69 ( Pt 1):219-26. doi: 10.1099/0022-1317-69-1-219.

Abstract

The nucleotide sequences of cDNA clones corresponding to the 3' OH end of papaya mosaic virus RNA have been determined. The 3'-terminal sequence obtained was 900 nucleotides in length, excluding the poly(A) tail, and contained an open reading frame capable of giving rise to a protein of 214 amino acid residues with an Mr of 22930. This protein was identified as the viral capsid protein. The 3' non-coding region of PMV genome RNA was about 121 nucleotides long [excluding the poly(A) tail] and homologous to the complementary sequence of the non-coding region at the 5' end of PMV RNA. A long open reading frame was also found in the predicted 5' end region of the negative strand.

摘要

已确定了与番木瓜花叶病毒RNA 3' OH末端相对应的cDNA克隆的核苷酸序列。所获得的3'末端序列长度为900个核苷酸,不包括聚(A)尾,并且包含一个开放阅读框,该阅读框能够产生一个由214个氨基酸残基组成、Mr为22930的蛋白质。该蛋白质被鉴定为病毒衣壳蛋白。PMV基因组RNA的3'非编码区约121个核苷酸长[不包括聚(A)尾],并且与PMV RNA 5'端非编码区的互补序列同源。在负链的预测5'端区域也发现了一个长开放阅读框。

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