Oral and Maxillofacial Surgery Department, Royal Brisbane and Women's Hospital, Herston, Queensland, Australia.
Saliva and Liquid Biopsy Translational Research Team, Institute of Health and Biomedical Innovation, School of Biomedical Science, Queensland University of Technology, Brisbane, Queensland, Australia.
Int J Oral Maxillofac Surg. 2021 Aug;50(8):994-998. doi: 10.1016/j.ijom.2020.11.012. Epub 2020 Dec 24.
Adenoid cystic carcinoma (ACC) is a rare salivary gland neoplasm with a poor long-term prognosis due to multiple recurrences and distant metastatic spread. Circulating tumour cells (CTCs) are tumour cells shed from a primary, recurrent, or metastatic cancer that are detectable in the blood or lymphatics. There is no literature to date confirming the presence of CTCs in ACC. The aim of this study was to determine whether CTCs are detectable in ACC. Blood samples were collected from eight patients with histologically confirmed ACC. The TNM stage of the tumour was recorded, as well as any prior treatment. CTCs were isolated by spiral microfluidics and detected by immunofluorescence staining. Three of the eight patients recruited (32.5%) had staining consistent with the presence of CTCs. Of these three patients with detectable CTCs, one had confirmed pulmonary metastasis, one had suspected pulmonary metastasis and was awaiting confirmation, and one had local recurrence confirmed on re-resection. One patient with known isolated pulmonary metastasis had previously undergone a lung metastasectomy and did not have CTCs detected. CTCs are detectable in ACC. In this small patient sample, CTCs were found to be present in those patients with recurrent local disease and known distant metastatic disease. CTCs in ACC should be investigated further for their potential use as an adjunct in staging, prognosis, and the detection of recurrence.
腺样囊性癌 (ACC) 是一种罕见的唾液腺肿瘤，由于多次复发和远处转移扩散，长期预后较差。循环肿瘤细胞 (CTC) 是从原发性、复发性或转移性癌症脱落的肿瘤细胞，可在血液或淋巴中检测到。目前尚无文献证实 ACC 中存在 CTC。本研究旨在确定 ACC 中是否可检测到 CTC。从 8 名经组织学证实患有 ACC 的患者中采集血样。记录肿瘤的 TNM 分期以及任何先前的治疗情况。CTC 通过螺旋微流控技术分离，并通过免疫荧光染色检测。招募的 8 名患者中有 3 名（32.5%）的染色结果与 CTC 的存在一致。在这 3 名可检测到 CTC 的患者中，1 名患者有确诊的肺转移，1 名患者有疑似肺转移，正在等待确认，1 名患者在再次切除后确认局部复发。1 名已知孤立性肺转移的患者之前已接受过肺转移切除术，未检测到 CTC。ACC 中可检测到 CTC。在这个小患者样本中，在那些局部复发和已知远处转移疾病的患者中发现了 CTC。ACC 中的 CTC 应进一步研究其作为分期、预后和复发检测的辅助手段的潜力。
