National Research Institute of Chinese Medicine, Ministry of Health and Welfare, 155-1 Li-Nung St., Sec. 2, Shipai, Peitou, Taipei 112, Taiwan.
National Research Institute of Chinese Medicine, Ministry of Health and Welfare, 155-1 Li-Nung St., Sec. 2, Shipai, Peitou, Taipei 112, Taiwan; Graduate Institute of Pharmacognosy, Taipei Medical University, 252 Wu-Hsing St., Taipei 110, Taiwan; Institute of Traditional Medicine, National Yang Ming Chiao Tung University, 155 Li-Nung St., 7 Sec. 2, Shipai, Beitou, Taipei 112, Taiwan; Department of Chinese Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Traditional Chinese Medicine Glycomics Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan. Electronic address: https://twitter.com/nricm.edu.tw.
Int J Biol Macromol. 2024 Oct;277(Pt 2):134360. doi: 10.1016/j.ijbiomac.2024.134360. Epub 2024 Jul 31.
Two novel sulfated polysaccharides (SPs), N10 and K5 were isolated from ammonium sulfate or potassium sulfate at concentrations of 10 mM and 5 mM in liquid cultures of Antrodia cinnamomea, respectively. N10 and K5 were galactoglucans with a galactose:glucose molar ratio of approximately 1:3. In lipopolysaccharide (LPS)-stimulated RAW264.7 cells, N10 and K5 exhibited strong anti-inflammatory potential, of 56 % and 23 % maximal inhibition of IL-6 and TNF-α production, respectively. Mechanical analysis revealed differences between N10 and K5, with N10 inhibiting the LPS-stimulated phosphorylation of ERK and p38 in RAW264.7 cells. K5 inhibited the LPS-stimulated phosphorylation of AKT and TGFβR-II. N10 and K5 were fragmented into F1, F2, and F3, the molecular weights of which were 455, 24, 0.9, and 327, 36, 1.9 kDa, respectively. K5 F2 and K5 F3 exhibited high degrees of sulfation of 1:3 and 1:8, resulting in strong anti-inflammation, of 83 % and 37 % highest inhibition of IL-6 and TNF-α production, respectively. Therefore, low-molecular-weight and high-sulfation-degree SPs exhibited strong anti-inflammatory activity. Specifically, K5 F2 inhibited the phosphorylation of p38, and K5 F3 suppressed the signaling pathway of p38/JNK. Overall, the sulfation degree of SPs is concluded to affect the anti-inflammatory responses.
两种新型硫酸化多糖（SPs），N10 和 K5，分别从金顶侧耳液体培养物中浓度为 10 mM 和 5 mM 的硫酸铵或硫酸钾中分离得到。N10 和 K5 是半乳糖葡聚糖，半乳糖：葡萄糖摩尔比约为 1:3。在脂多糖（LPS）刺激的 RAW264.7 细胞中，N10 和 K5 表现出强烈的抗炎潜力，分别最大抑制 IL-6 和 TNF-α 产生的 56%和 23%。力学分析表明 N10 和 K5 之间存在差异，N10 抑制 LPS 刺激的 RAW264.7 细胞中 ERK 和 p38 的磷酸化。K5 抑制 LPS 刺激的 AKT 和 TGFβR-II 的磷酸化。N10 和 K5 被片段化为 F1、F2 和 F3，分子量分别为 455、24、0.9 和 327、36、1.9 kDa。K5 F2 和 K5 F3 具有 1:3 和 1:8 的高硫酸化程度，表现出很强的抗炎作用，最大抑制 IL-6 和 TNF-α 产生的分别为 83%和 37%。因此，低分子量和高硫酸化程度的 SPs 表现出很强的抗炎活性。具体来说，K5 F2 抑制了 p38 的磷酸化，而 K5 F3 抑制了 p38/JNK 信号通路。总体而言，SPs 的硫酸化程度影响抗炎反应。
