Directorate General for Disease Surveillance and Control, Ministry of Health, Sultanate of Oman, P.O. Box 393, PC 100 Muscat, Oman.
Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, PC 616 Nizwa, Oman.
Int J Infect Dis. 2021 Mar;104:139-149. doi: 10.1016/j.ijid.2020.12.049. Epub 2021 Jan 13.
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been proven to be lethal to human health, which affects almost every corner of the world. The objectives of this study were to add context to the global data and international genomic consortiums, and to give insight into the efficiency of the contact tracing system in Oman.
We combined epidemiological data and whole-genome sequence data from 94 samples of SARS-CoV-2 in Oman to understand the origins, genetic variation, and transmissibility. The whole-genome size of sequence data was obtained through a customized SARS-COV-2 research panel. Amplifier methods ranged from 26 Kbp to 30 Kbp and were submitted to GISAID.
The study found that P323L (94.7%) is the most common mutation, followed by D614G (92.6%) Spike protein mutation. A unique mutation, I280V, was first reported in Oman and was associated with a rare lineage, B.1.113 (10.6%). In addition, the study revealed a good agreement between genetic and epidemiological data.
Oman's robust surveillance system was very efficient in guiding the outbreak investigation processes in the country, the study illustrates the future importance of molecular epidemiology in leading the national response to outbreaks and pandemics.
严重急性呼吸综合征冠状病毒 2 型（SARS-CoV-2）已被证明对人类健康具有致命性，其影响几乎遍及世界的每一个角落。本研究的目的是为全球数据和国际基因组联盟提供背景信息，并深入了解阿曼接触者追踪系统的效率。
我们结合了 94 份阿曼 SARS-CoV-2 样本的流行病学数据和全基因组序列数据，以了解其起源、遗传变异和传染性。通过定制的 SARS-COV-2 研究面板获得全基因组序列数据的大小。扩增方法的范围从 26 Kbp 到 30 Kbp，并提交给 GISAID。
研究发现，P323L（94.7%）是最常见的突变，其次是 D614G（92.6%）棘突蛋白突变。在阿曼首次报告了一种独特的突变 I280V，与罕见的谱系 B.1.113（10.6%）有关。此外，该研究还揭示了遗传和流行病学数据之间的良好一致性。
阿曼强大的监测系统在指导该国的疫情调查过程中非常有效，该研究说明了分子流行病学在引领国家应对疫情和大流行方面的未来重要性。
