Antimicrobial capacity and physico-chemical characteristics of adhesive resin containing riboflavin after photodynamic therapy.

PURPOSE

To assess the micro-tensile bond strength (μ-TBS), degree of conversion, and antimicrobial capacity of modified dentin adhesive using riboflavin photoinitiators.

MATERIALS AND METHODS

Three groups of adhesives (control; 0.1 % riboflavin-PDT and 0.5 % riboflavin PDT) were prepared and tested on 70 sound molar teeth. Fourier-transformed infrared spectroscopy (FTIR) was performed for riboflavin and riboflavin-modified adhesives to calculate the degree of conversion. Contact angle measurement was performed by dropping a 5 μL droplet of the adhesives onto polished dentin disk. For μ-TBS testing, the dentin beams were fixed on a micro-tensile tester and were loaded in tension at a cross-head speed of 1 mm per min until fracture. The viability of Streptococcus mutans biofilm was tested using MTT assay.

RESULTS

The spectrum at 1728 cm-1 indicates CO stretching frequency of riboflavin. The groups control and 0.1 % riboflavin PDT showed the highest degree of conversion followed by lowest degree of conversion of 0.5 % riboflavin adhesive after PDT. The control adhesive exhibited the highest contact angle over the dentin surface compared to the riboflavin-modified adhesive groups (p < 0.05). The lowest contact angle was observed for 0.1 % riboflavin that showed increased permeability of the adhesive onto the dentin surface (p < 0.05). The variables of immediate bonding (F = 12.328, p = 0.000) and ageing in artificial saliva (F = 41.559, p = 0.000) significantly affected the bond strength. For aged μ-TBS testing, the scores for 0.1 % riboflavin PDT was significantly higher as compared to the 0.5 % riboflavin PDT samples. The MTT resulted in a lower S. mutans viability for 0.5 % riboflavin PDT compared to 0.1 % riboflavin PDT and control adhesive (p < 0.05), irrespective of any time point, that is, after 24 h and 30 days ageing.

CONCLUSIONS

The addition of riboflavin as photosensitizer in dentin adhesive demonstrated higher bond strength, excellent antimicrobial capability, and degree of conversion following PDT. The addition of riboflavin in dentin adhesive for PDT could be used as a potential restorative material in adhesive dentistry.