Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2492, Rio Branco, 90035-003, Porto Alegre, RS, Brazil.
Department of Microbiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500, Centro, 90050-170, Porto Alegre, RS, Brazil.
J Dent. 2019 Apr;83:33-39. doi: 10.1016/j.jdent.2019.02.002. Epub 2019 Feb 19.
The aim of this study was to formulate and to evaluate the immediate and long-term physical, chemical and antibacterial properties of an experimental adhesive resin with chitosan or triclosan-loaded chitosan.
Chitosan, triclosan and triclosan-loaded chitosan were evaluated for scanning electron microscopy (SEM) and micro-Raman spectroscopy. An experimental adhesive resin was formulated with methacrylate monomers and photoinitiators. Chitosan or triclosan-loaded chitosan were added at 2 (G and G) or 5 (G and G) wt.% in the base resin. The base resin was used as control (G). The adhesives were evaluated for degree of conversion (DC), Knoop hardness (KHN), softening in solvent (ΔKHN), immediate and long-term microtensile bond-strength (μ-TBS) and antibacterial activity.
SEM indicated triclosan sticks and chitosan porosity. Triclosan-loaded chitosan presented structures on chitosan. Micro-Raman indicated no chemical interaction between chitosan and triclosan. There was no difference among groups for DC (p > 0.05). Initial KHN ranged from 17.36 (±1.56) to 20.38 (±1.72), with higher value for G compared to G (p < 0.05). G presented the lowest ΔKHN% (p < 0.05). There were no differences in the immediate or long-term μ-TBS (p > 0.05). G and G decreased the μ-TBS after storage (p < 0.05). Chitosan groups showed higher biofilm formation (p < 0.05). Triclosan-loaded chitosan groups presented lower biofilm formation (p < 0.05). There was no activity against planktonic bacteria regardless the time of evaluation (p > 0.05).
Triclosan-loaded chitosan at 5 wt.% addition in an experimental adhesive resin showed reliable properties, with the highest antibacterial activity immediately and after six months, and induced dentin/adhesive interface stability over time.
Triclosan-loaded chitosan groups showed antibacterial activity immediately and over time and induced dentin/adhesive interface stability, may positively affecting long-lasting marginal sealing.
本研究旨在制备并评价一种含有壳聚糖或载有三氯生的壳聚糖的实验性黏附树脂的即时和长期物理、化学和抗菌性能。
通过扫描电子显微镜（SEM）和微拉曼光谱对壳聚糖、三氯生和载有三氯生的壳聚糖进行评估。用甲基丙烯酸酯单体和光引发剂制备实验性黏附树脂。壳聚糖或载有三氯生的壳聚糖以 2（G 和 G）或 5（G 和 G）wt.%的比例添加到基础树脂中。基础树脂用作对照（G）。评估了黏合剂的转化率（DC）、努普硬度（KHN）、溶剂软化（ΔKHN）、即时和长期微拉伸结合强度（μ-TBS）和抗菌活性。
SEM 表明三氯生棒和壳聚糖的多孔性。载有三氯生的壳聚糖呈现出壳聚糖上的结构。微拉曼表明壳聚糖和三氯生之间没有化学相互作用。各组间的 DC 无差异（p>0.05）。初始 KHN 范围为 17.36（±1.56）至 20.38（±1.72），G 比 G 具有更高的值（p<0.05）。G 组的 ΔKHN%最低（p<0.05）。即时和长期的 μ-TBS 无差异（p>0.05）。G 和 G 组在储存后降低了 μ-TBS（p<0.05）。壳聚糖组显示出更高的生物膜形成（p<0.05）。载有三氯生的壳聚糖组显示出较低的生物膜形成（p<0.05）。无论评估时间如何，都没有对浮游细菌的活性（p>0.05）。
在实验性黏附树脂中添加 5wt.%的载有三氯生的壳聚糖具有可靠的性能，具有最高的即时和六个月后的抗菌活性，并随着时间的推移诱导牙本质/黏合剂界面稳定性。
载有三氯生的壳聚糖组具有即时和长期的抗菌活性，并诱导牙本质/黏合剂界面稳定性，可能对长期边缘密封产生积极影响。
