电刺激可促进小鼠腭部伤口的早期愈合。
Electrical stimulation enhances early palatal wound healing in mice.
作者信息
Ferreira Camila Lopes, Neves Jardini Maria Aparecida, Moretto Nunes Camilla Magnoni, Bernardo Daniella Vicensotto, Viana Casarin Renato Corrêa, Dos Santos Gedraite Estevão, Mathias Márcio Antônio, Liu Fei, Mendonça Gustavo, Silveira Mendonça Daniela Baccelli, Santamaria Mauro Pedrine
机构信息
Postgraduate Student, Oral Biopathology Program, Periodontics Area, São Paulo State University (UNESP), College of Dentistry, São José dos Campos, Brazil.
São Paulo State University (UNESP), Division of Periodontics, Institute of Science and Technology, São José dos Campos, São Paulo, Brazil.
出版信息
Arch Oral Biol. 2021 Feb;122:105028. doi: 10.1016/j.archoralbio.2020.105028. Epub 2020 Dec 20.
BACKGROUND/OBJECTIVE: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice.
METHODS
Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method.
RESULTS
Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups.
CONCLUSION
ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.
背景/目的：电刺激（ES）已被用于治疗慢性伤口及其他临床应用，在伤口愈合方面显示出良好效果。据推测，ES对口腔黏膜愈合可能具有积极作用。本研究旨在探讨ES对瑞士小鼠腭黏膜早期愈合过程的影响。
方法
将90只动物分为两组：对照组（C；n = 45），接受假电刺激；试验组（ES；n = 45），每天接受一次ES（100 μA；9 kHz；660 mVpp），共3天。用直径1.5 mm的活检打孔器在硬腭上制作全层伤口。组织学上，评估以下参数：腭部伤口闭合情况以及上皮和结缔组织伤口边缘距离（EED和CED）。此外，通过多重检测法评估白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）和血管内皮生长因子（VEGF）细胞因子水平。使用偏振光法评估胶原纤维百分比，使用免疫荧光法评估Smad蛋白。
结果
ES组在第5天时腭部伤口闭合情况有显著改善（p = 0.01）。此外，ES组在所有时间点的EED和CED均较短（p < 0.05），炎症标志物IL-6、IL-10、TNF-α和VEGF水平降低（p < 0.05）。两组之间胶原纤维和磷酸化Smad2无差异。
结论
ES对腭部伤口早期闭合结果以及炎症标志物有积极作用。
Zotero 插件