Anhui Provincial Key Laboratory of Environmental Pollution Control and Resource Reuse, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei, China.
Anhui Provincial Key Laboratory of Environmental Pollution Control and Resource Reuse, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei, China.
Sci Total Environ. 2021 Mar 25;762:144180. doi: 10.1016/j.scitotenv.2020.144180. Epub 2020 Dec 17.
Toxicity interaction, synergism and antagonism, may occur when multiple pollutants are exposed to the environment simultaneously, which limits the utility of some standard models to assess toxicity hazards and risks. The development and application of models which can provide an insight into the combined toxicity of pollutants becomes necessary. Therefore, a novel model, area-concentration ratio (ACR) method, was developed to characterize the toxicity interaction within mixtures of three aminoglycoside antibiotics (AGs), kanamycin sulfate (KAN), paromomycin sulfate (PAR), tobramycin (TOB) and one heavy metal copper (Cu) in this study. The inhibition toxicity of single contaminants and mixtures designed by direct equilibration ray method and uniform design ray method to Chlorella pyrenoidosa (C. pyrenoidosa) was determined by the microplate toxicity analysis (MTA). The results showed that the novel method ACR could be used for quantitative characterization of combined toxicity. According to the ACR, all the binary AG antibiotic mixture systems display obvious synergism and weak antagonism. The addition of the heavy metal Cu into binary AG antibiotic mixtures can obviously change toxicity interaction, but toxicity interaction changing trend varies greatly in different ternary mixture systems. Toxicity interaction in the six mixture systems has component concentration-ratio dependence. ACR can be suggested as an effective novel method to quantitatively characterize toxicity interaction when assessing the hazards and risks of the combined pollution.
当多种污染物同时暴露于环境中时，可能会发生毒性相互作用、协同作用和拮抗作用，这限制了一些标准模型在评估毒性危害和风险方面的应用。因此，有必要开发和应用能够深入了解污染物联合毒性的模型。因此，本研究开发了一种新的模型，即面积-浓度比（ACR）方法，用于表征三种氨基糖苷抗生素（AGs）硫酸卡那霉素（KAN）、硫酸巴龙霉素（PAR）、妥布霉素（TOB）和一种重金属铜（Cu）混合物中污染物的毒性相互作用。通过微孔板毒性分析（MTA）测定了直接平衡射线法和均匀设计射线法设计的单一污染物和混合物对栅藻（Chlorella pyrenoidosa）的抑制毒性。结果表明，新型 ACR 方法可用于定量表征联合毒性。根据 ACR，所有二元 AG 抗生素混合物系统均表现出明显的协同作用和较弱的拮抗作用。重金属 Cu 的加入会明显改变二元 AG 抗生素混合物的毒性相互作用，但在不同的三元混合物系统中，毒性相互作用的变化趋势差异很大。六个混合物系统中的毒性相互作用具有组分浓度比依赖性。ACR 可以作为一种有效的新方法，用于评估联合污染的危害和风险时定量表征毒性相互作用。
