Guangxi Engineering Technology Research Center of Chinese Medicinal Materials Stock Breeding, Guangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi, China.
College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, China.
PLoS One. 2020 Dec 23;15(12):e0243778. doi: 10.1371/journal.pone.0243778. eCollection 2020.
The giant freshwater prawn, Macrobrachium rosenbergii (M. rosenbergii) as an important freshwater aquaculture species with high commercial value, exhibited unsynchronized growth. However, the potentially metabolic mechanism remains unclear. In this study, we used liquid chromatography tandem mass spectrometry (LC-MS/MS) to investigate the hepatopancreatic metabolic profiles of twenty giant freshwater prawns between the fast-growing group and slow-growing group. In the metabolomics assay, we isolated 8,293 peaks in positive and negative iron mode. Subsequently, 44 significantly differential metabolites were identified. Functional pathway analysis revealed that these metabolites were significantly enriched in three key metabolic pathways. Further integrated analysis indicated that glycerophospholipid metabolism and aminoacyl-tRNA biosynthesis have significant impact on growth performance in M.rosenbergii. Our findings presented here demonstrated the critical metabolites and metabolic pathways involved in growth performance, moreover provided strong evidence for elucidating the potentially metabolic mechanism of the unsynchronized growth in M. rosenbergii.
巨型淡水虾，罗氏沼虾（Macrobrachium rosenbergii）作为一种具有高商业价值的重要淡水养殖物种，表现出生长不同步的现象。然而，其潜在的代谢机制尚不清楚。在这项研究中，我们使用液相色谱-串联质谱（LC-MS/MS）技术研究了快速生长组和缓慢生长组的 20 只巨型淡水虾的肝胰腺代谢谱。在代谢组学分析中，我们在正、负离子模式下分离出 8293 个峰。随后，鉴定出 44 个显著差异的代谢物。功能途径分析表明，这些代谢物在三个关键代谢途径中显著富集。进一步的综合分析表明，甘油磷脂代谢和氨酰-tRNA 生物合成对罗氏沼虾的生长性能有重要影响。本研究结果表明，参与生长性能的关键代谢物和代谢途径，为阐明罗氏沼虾生长不同步的潜在代谢机制提供了有力证据。
