Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215006, China; Department of Spinal Surgery, Traditional Chinese Medicine Hospital of Kunshan Affiliated to Nanjing TCM University Kunshan, 215300, China.
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.
Int J Biol Macromol. 2018 Oct 1;117:144-151. doi: 10.1016/j.ijbiomac.2018.05.175. Epub 2018 May 24.
Silk porous scaffolds have shown promising applications in tissue regenerations as cellular scaffolds to incorporate cells in vitro and in vivo, and facilitate cell proliferation and production of extracellular matrix. It remains strong needs to optimize the microstructures and performances of silk scaffolds for better biocompatibility. Here, a green process was developed to form water-insoluble scaffolds. Repeated freezing-dissolving procedures and silk nanofibers were introduced to tune the performances of the scaffolds, resulting in amorphous conformations and nanofibrous structures. Controllable degradation and mechanical properties as well as improved cell compatibility were then achieved for these scaffolds, suggesting their promising future in tissue regenerations. Our present results confirmed the possibility of actively designing silk scaffolds with preferable properties used in various tissue regenerations.
丝素多孔支架作为细胞支架，在组织再生中具有良好的应用前景，可用于体外和体内培养细胞，并促进细胞增殖和细胞外基质的产生。因此，仍强烈需要优化丝素支架的微观结构和性能，以提高其生物相容性。本研究开发了一种形成不溶性支架的绿色工艺。通过重复的冻融处理和丝素纳米纤维的引入，对支架的性能进行了调控，得到了无定形结构和纳米纤维结构。这些支架具有可控的降解性能和力学性能，以及更好的细胞相容性，这表明它们在组织再生中有很好的应用前景。本研究结果证实了通过主动设计具有所需性能的丝素支架，用于各种组织再生的可能性。
