Widayati Diah Tri, Pangestu Mulyoto
Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Department of Animal Reproduction, Faculty of Animal Science, Jenderal Soedirman, Purwokerto, Central Java 53122 Indonesia.
Vet World. 2020 Nov;13(11):2443-2446. doi: 10.14202/vetworld.2020.2443-2446. Epub 2020 Nov 14.
Bligon goat is a crossbreed between Etawah and Kacang goat. This crossbreed goat is mostly reared by small farmers. maturation allows female goat (does) contributes toward reproduction despite the fact that the animal has been slaughtered. The aim of this study was to determine the maturation rate of Bligon goat oocytes supplemented with follicle-stimulating hormone (FSH), and their ability for further embryonic development after fertilization.
Experiment was conducted at the Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, using Bligon goat ovaries obtained from local slaughterhouse around Yogyakarta. One thousand five hundred cumulus-oocyte complexes were matured for 24 h in tissue culture medium 199 supplemented with 50 IU/L FSH or without FSH (control). First, matured oocytes were evaluated its morphology based on the expansion of cumulus cells and PB1 extrusion. Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. Oocytes were considered mature when they reached metaphase II. To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization . The data were analyzed using analysis of variance.
The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. The rate of degeneration was lower in the FSH-supplemented medium (3.21±0.25 vs. 10.17±3.15%). The blastocyst stage of oocyte developed embryos was reached by 12.43±2.15% and 22.28±4.86% of the control and treatment groups, respectively.
FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development .
布利贡山羊是埃塔瓦山羊和卡仓山羊的杂交品种。这种杂交山羊大多由小农户饲养。尽管动物已被屠宰，但成熟可使雌性山羊（母羊）参与繁殖。本研究的目的是确定添加促卵泡激素（FSH）的布利贡山羊卵母细胞的成熟率，以及它们受精后进一步胚胎发育的能力。
实验在日惹加查马达大学动物科学学院动物生理学与繁殖实验室进行，使用从日惹周边当地屠宰场获取的布利贡山羊卵巢。1500个卵丘 - 卵母细胞复合体在添加50 IU/L FSH的组织培养液199中或不添加FSH（对照）的情况下成熟24小时。首先，根据卵丘细胞的扩展和第一极体（PB1）的排出评估成熟卵母细胞的形态。接下来，600个卵母细胞用1% 醋酸洋红染色，根据染色体构型变化和核膜破裂情况检查成熟度。当卵母细胞达到减数第二次分裂中期时被认为成熟。为证明成熟卵母细胞发育成胚胎的能力，对900个卵母细胞进行受精处理。数据采用方差分析。
结果表明，添加FSH显著提高了卵母细胞成熟率（分别为65.21±7.26% 和43.25±6.23%），这由PB1排出以及同源染色体配对并排列在赤道上表明。在添加FSH的培养基中退化率较低（分别为3.21±0.25% 和10.17±3.15%）。对照组和处理组卵母细胞发育成胚胎的囊胚阶段分别达到12.43±2.15% 和22.28±4.86%。
添加FSH显著提高卵母细胞成熟度，并产生成熟卵母细胞用于未来的胚胎发育。
