Institute of Animal Physiology and Genetics, Czech Academy of Science, Libechov, Czech Republic.
Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague 2, Czech Republic.
Biol Reprod. 2019 Oct 25;101(4):719-732. doi: 10.1093/biolre/ioz117.
Culture media used in assisted reproduction are commonly supplemented with gonadotropin hormones to support the nuclear and cytoplasmic maturation of in vitro matured oocytes. However, the effect of gonadotropins on protein synthesis in oocytes is yet to be fully understood. As published data have previously documented a positive in vitro effect of follicle-stimulating hormone (FSH) on cytoplasmic maturation, we exposed mouse denuded oocytes to FSH in order to evaluate the changes in global protein synthesis. We found that dose-dependent administration of FSH resulted in a decrease of methionine incorporation into de novo synthesized proteins in denuded mouse oocytes and oocytes cultured in cumulus-oocyte complexes. Similarly, FSH influenced methionine incorporation in additional mammalian species including human. Furthermore, we showed the expression of FSH-receptor protein in oocytes. We found that major translational regulators were not affected by FSH treatment; however, the amino acid uptake became impaired. We propose that the effect of FSH treatment on amino acid uptake is influenced by FSH receptor with the effect on oocyte metabolism and physiology.
辅助生殖中使用的培养基通常补充有促性腺激素，以支持体外成熟卵母细胞的核和细胞质成熟。然而，促性腺激素对卵母细胞中蛋白质合成的影响尚未被完全理解。由于之前发表的数据证明了卵泡刺激素 (FSH) 在细胞质成熟方面有体外的积极作用，我们用 FSH 处理小鼠去卵丘卵母细胞，以评估整体蛋白质合成的变化。我们发现，FSH 的剂量依赖性给药导致去卵丘的小鼠卵母细胞和在卵丘-卵母细胞复合物中培养的卵母细胞中新合成蛋白质的蛋氨酸掺入减少。同样，FSH 也影响了包括人类在内的其他哺乳动物物种的蛋氨酸掺入。此外，我们还在卵母细胞中显示了 FSH 受体蛋白的表达。我们发现，主要的翻译调节剂不受 FSH 处理的影响；然而，氨基酸摄取受到了损害。我们提出，FSH 处理对氨基酸摄取的影响受 FSH 受体的影响，进而影响卵母细胞的代谢和生理功能。
