Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
Front Immunol. 2020 Dec 8;11:614972. doi: 10.3389/fimmu.2020.614972. eCollection 2020.
Human induced Pluripotent Stem Cell (hiPSC) models are a valuable new tool for research into neurodegenerative diseases. Neuroinflammation is now recognized as a key process in neurodegenerative disease and aging, and microglia are central players in this. A plethora of hiPSC-derived microglial models have been published recently to explore neuroinflammation, ranging from monoculture through to xenotransplantation. However, combining physiological relevance, reproducibility, and scalability into one model is still a challenge. We examine key features of the microglial environment, especially media composition, extracellular matrix, and co-culture, to identify areas for improvement in current hiPSC-microglia models.
人诱导多能干细胞（hiPSC）模型是研究神经退行性疾病的一种有价值的新工具。神经炎症现在被认为是神经退行性疾病和衰老的一个关键过程，小胶质细胞是其中的核心参与者。最近已经发表了大量的 hiPSC 衍生的小胶质细胞模型来探索神经炎症，从单核培养到异种移植不等。然而，将生理相关性、可重复性和可扩展性结合到一个模型中仍然是一个挑战。我们研究了小胶质细胞环境的关键特征，特别是培养基组成、细胞外基质和共培养，以确定当前 hiPSC-小胶质细胞模型的改进领域。
