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微小锥虫衍生RNA和锥虫mRNA的帽结构均为7-甲基鸟苷。

The cap of both miniexon-derived RNA and mRNA of trypanosomes is 7-methylguanosine.

作者信息

Sutton R E, Boothroyd J C

机构信息

Department of Medical Microbiology, Stanford University School of Medicine, California 94305.

出版信息

Mol Cell Biol. 1988 Jan;8(1):494-6. doi: 10.1128/mcb.8.1.494-496.1988.

DOI:10.1128/mcb.8.1.494-496.1988
PMID:3336365
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363158/
Abstract

Most, if not all, trypanosome mRNAs have the same 35-base sequence at their 5' terminus which is derived from a short RNA (medRNA) probably by the process of trans-splicing. It is of interest, evolutionarily and mechanistically, to determine the chemical structure of the 5' terminus of the precursor (medRNA) and product (mRNA). We demonstrate here that the cap structure of both is most probably 7-methylguanosine in a 5',5' triphosphate linkage, consistent with a precursor/product relationship.

摘要

大多数（如果不是全部的话）锥虫mRNA在其5'末端具有相同的35个碱基的序列，该序列可能通过反式剪接过程源自短RNA（medRNA）。从进化和机制的角度确定前体（medRNA）和产物（mRNA）5'末端的化学结构是很有意义的。我们在此证明两者的帽结构很可能是5',5'三磷酸连接的7-甲基鸟苷，这与前体/产物关系一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/363158/6b9226daba41/molcellb00061-0517-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/363158/4447ac0055c0/molcellb00061-0516-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/363158/6b9226daba41/molcellb00061-0517-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/363158/4447ac0055c0/molcellb00061-0516-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/363158/6b9226daba41/molcellb00061-0517-a.jpg

相似文献

1
The cap of both miniexon-derived RNA and mRNA of trypanosomes is 7-methylguanosine.微小锥虫衍生RNA和锥虫mRNA的帽结构均为7-甲基鸟苷。
Mol Cell Biol. 1988 Jan;8(1):494-6. doi: 10.1128/mcb.8.1.494-496.1988.
2
Direct analysis of the mini-exon donor RNA of Trypanosoma brucei: detection of a novel cap structure also present in messenger RNA.布氏锥虫微小外显子供体RNA的直接分析：信使RNA中也存在的一种新型帽结构的检测
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3
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Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA.锥虫中的反式剪接需要剪接前导RNA 5'端的甲基化。
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引用本文的文献

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The cAMP-specific phosphodiesterase TbPDE2C is an essential enzyme in bloodstream form Trypanosoma brucei.环磷酸腺苷特异性磷酸二酯酶TbPDE2C是布氏锥虫血流形式中的一种必需酶。
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2
Trypanosome trans-splicing utilizes 2'-5' branches and a corresponding debranching activity.锥虫的反式剪接利用2'-5'分支和相应的去分支活性。
EMBO J. 1988 May;7(5):1431-7. doi: 10.1002/j.1460-2075.1988.tb02960.x.
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Polycistronic transcripts in trypanosomes and their accumulation during heat shock: evidence for a precursor role in mRNA synthesis.

本文引用的文献

1
Transcripts coding for variant surface glycoproteins of Trypanosoma brucei have a short, identical exon at their 5' end.编码布氏锥虫可变表面糖蛋白的转录本在其5'端有一个短的、相同的外显子。
Gene. 1982 Dec;20(2):281-9. doi: 10.1016/0378-1119(82)90046-4.
2
Sequences homologous to variant antigen mRNA spliced leader in Trypanosomatidae which do not undergo antigenic variation.与锥虫科中不发生抗原变异的可变抗原mRNA剪接前导序列同源的序列。
Nature. 1984;308(5960):665-7. doi: 10.1038/308665a0.
3
Sequences homologous to the variant antigen mRNA spliced leader are located in tandem repeats and variable orphons in trypanosoma brucei.
锥虫中的多顺反子转录本及其在热休克期间的积累：mRNA合成中前体作用的证据。
Mol Cell Biol. 1988 Sep;8(9):3837-46. doi: 10.1128/mcb.8.9.3837-3846.1988.
4
A 5' exo-ribonuclease and RNA ligase of T. brucei.布氏锥虫的一种5'外切核糖核酸酶和RNA连接酶。
Nucleic Acids Res. 1988 Oct 25;16(20):9737-59. doi: 10.1093/nar/16.20.9737.
5
Permeable trypanosome cells as a model system for transcription and trans-splicing.可渗透的锥虫细胞作为转录和反式剪接的模型系统。
Nucleic Acids Res. 1990 Jun 11;18(11):3319-26. doi: 10.1093/nar/18.11.3319.
6
A spliced leader is present on a subset of mRNAs from the human parasite Schistosoma mansoni.剪接前导序列存在于人类寄生虫曼氏血吸虫的一部分mRNA中。
Proc Natl Acad Sci U S A. 1990 Nov;87(22):8879-83. doi: 10.1073/pnas.87.22.8879.
7
In vitro capping in Trypanosoma cruzi identifies and shows specificity for the spliced leader RNA and U-RNAs.克氏锥虫中的体外加帽作用鉴定出剪接前导RNA和U-RNA并显示出特异性。
Nucleic Acids Res. 1990 Jul 25;18(14):4197-206. doi: 10.1093/nar/18.14.4197.
8
trans-spliced Caenorhabditis elegans mRNAs retain trimethylguanosine caps.经反式剪接的秀丽隐杆线虫信使核糖核酸保留三甲基鸟苷帽结构。
Mol Cell Biol. 1990 Apr;10(4):1764-8. doi: 10.1128/mcb.10.4.1764-1768.1990.
9
RNA-protein complexes mediate in vitro capping of the spliced-leader primary transcript and U-RNAs in Trypanosoma cruzi.RNA-蛋白质复合物介导克氏锥虫中剪接前导序列初级转录本和U-RNA的体外加帽。
Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5626-30. doi: 10.1073/pnas.88.13.5626.
10
Characterization of VSG gene expression site promoters and promoter-associated DNA rearrangement events.VSG基因表达位点启动子及启动子相关DNA重排事件的特征分析
Mol Cell Biol. 1991 May;11(5):2467-80. doi: 10.1128/mcb.11.5.2467-2480.1991.
与变异抗原mRNA剪接前导序列同源的序列位于布氏锥虫的串联重复序列和可变孤儿基因中。
Cell. 1983 Oct;34(3):901-9. doi: 10.1016/0092-8674(83)90547-0.
4
Tandem repetition of the 5' mini-exon of variant surface glycoprotein genes: a multiple promoter for VSG gene transcription?变异表面糖蛋白基因5' 小外显子的串联重复：VSG基因转录的多个启动子？
Cell. 1983 Oct;34(3):891-900. doi: 10.1016/0092-8674(83)90546-9.
5
5'-terminal cap structure in eucaryotic messenger ribonucleic acids.真核生物信使核糖核酸中的5'-末端帽结构。
Microbiol Rev. 1980 Jun;44(2):175-205. doi: 10.1128/mr.44.2.175-205.1980.
6
Comparison of the genes coding for the common 5' terminal sequence of messenger RNAs in three trypanosome species.三种锥虫物种中信使核糖核酸常见5'末端序列编码基因的比较。
Nucleic Acids Res. 1984 Jun 11;12(11):4431-43. doi: 10.1093/nar/12.11.4431.
7
Yeast contains small nuclear RNAs encoded by single copy genes.酵母含有由单拷贝基因编码的小核RNA。
Cell. 1983 Dec;35(3 Pt 2):743-51. doi: 10.1016/0092-8674(83)90107-1.
8
SnRNAs, SnRNPs, and RNA processing.小核RNA、小核核糖核蛋白与RNA加工
Annu Rev Biochem. 1982;51:617-54. doi: 10.1146/annurev.bi.51.070182.003153.
9
The chromatography of RNA and oligoribonucleotides on boronate-substituted agarose and polyacrylamide.RNA和寡核糖核苷酸在硼酸酯取代的琼脂糖和聚丙烯酰胺上的色谱分析。
Anal Biochem. 1980 Sep 1;107(1):128-35. doi: 10.1016/0003-2697(80)90502-3.
10
Discontinuous synthesis of mRNA in trypanosomes.锥虫中mRNA的不连续合成。
EMBO J. 1984 Oct;3(10):2387-92. doi: 10.1002/j.1460-2075.1984.tb02144.x.