锥虫中反式剪接的证据。
Evidence for trans splicing in trypanosomes.
作者信息
Sutton R E, Boothroyd J C
出版信息
Cell. 1986 Nov 21;47(4):527-35. doi: 10.1016/0092-8674(86)90617-3.
Abstract
The 5' ends of trypanosome mRNAs consist of an identical sequence of 35 nucleotides. This "mini-exon" sequence is derived from the 5' end of a 137 nucleotide RNA (medRNA). The remainder of each mRNA is derived from a protein-coding exon that is not linked to the mini-exon. We propose that medRNA is spliced in trans to de-novo-initiated transcripts of protein-coding genes. This trans splicing model predicts that the downstream portion of medRNA will be part of a branched structure and then be released as a free product (minRNA). We demonstrate that significant levels of minRNA exist in trypanosome RNA. Furthermore, minRNA can be released from high molecular weight RNA by a HeLa cell S100 "debranching" extract. We conclude that trans splicing is the physiological process by which mature mRNA molecules are synthesized in trypanosomes.
摘要
锥虫mRNA的5'端由35个核苷酸的相同序列组成。这个“微型外显子”序列来自于一个137个核苷酸RNA（medRNA）的5'端。每个mRNA的其余部分来自于一个与微型外显子不相连的蛋白质编码外显子。我们提出，medRNA通过反式剪接与蛋白质编码基因的从头起始转录本结合。这个反式剪接模型预测，medRNA的下游部分将成为分支结构的一部分，然后作为游离产物（minRNA）释放出来。我们证明，锥虫RNA中存在大量的minRNA。此外，minRNA可以通过HeLa细胞S100“去分支”提取物从高分子量RNA中释放出来。我们得出结论，反式剪接是锥虫中合成成熟mRNA分子的生理过程。
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本文引用的文献
1
Transcripts coding for variant surface glycoproteins of Trypanosoma brucei have a short, identical exon at their 5' end.编码布氏锥虫可变表面糖蛋白的转录本在其5'端有一个短的、相同的外显子。
Gene. 1982 Dec;20(2):281-9. doi: 10.1016/0378-1119(82)90046-4.
2
Sequences homologous to variant antigen mRNA spliced leader in Trypanosomatidae which do not undergo antigenic variation.与锥虫科中不发生抗原变异的可变抗原mRNA剪接前导序列同源的序列。
Nature. 1984;308(5960):665-7. doi: 10.1038/308665a0.
3
Two modes of activation of a single surface antigen gene of Trypanosoma brucei.布氏锥虫单个表面抗原基因的两种激活模式。
Cell. 1984 Jan;36(1):163-70. doi: 10.1016/0092-8674(84)90085-0.
4
Many trypanosome messenger RNAs share a common 5' terminal sequence.许多锥虫信使核糖核酸共享一个共同的5'末端序列。
Nucleic Acids Res. 1984 May 11;12(9):3777-90. doi: 10.1093/nar/12.9.3777.
5
Chromosome rearrangements in Trypanosoma brucei.布氏锥虫中的染色体重排
Cell. 1984 Nov;39(1):213-21. doi: 10.1016/0092-8674(84)90207-1.
6
Characterization of the 1.35 kilobase DNA repeat unit containing the conserved 35 nucleotides at the 5'-termini of variable surface glycoprotein mRNAs in Trypanosoma brucei.布氏锥虫可变表面糖蛋白mRNA 5'末端含保守35个核苷酸的1.35千碱基DNA重复单元的特征分析
Nucleic Acids Res. 1984 Jun 25;12(12):4907-20. doi: 10.1093/nar/12.12.4907.
7
Gene activation and re-expression of a Trypanosoma brucei variant surface glycoprotein.布氏锥虫变异表面糖蛋白的基因激活与重新表达
EMBO J. 1983;2(7):1185-92. doi: 10.1002/j.1460-2075.1983.tb01565.x.
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9
Tandem repetition of the 5' mini-exon of variant surface glycoprotein genes: a multiple promoter for VSG gene transcription?变异表面糖蛋白基因5' 小外显子的串联重复：VSG基因转录的多个启动子？
Cell. 1983 Oct;34(3):891-900. doi: 10.1016/0092-8674(83)90546-9.
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