Kourousekos Georgios D, Theodosiadou Ekaterini K, Lymberopoulos Aristotelis G, Belibasaki Sofia, Boscos Constantin
Faculty of Veterinary Medicine, , , .
Faculty of Veterinary Medicine, University of Thessaly, 43100, Karditsa, .
Anim Reprod. 2018 Aug 16;15(1):75-83. doi: 10.21451/1984-3143-2017-AR939.
The effect of prolonged aflatoxin B (AFB) administration on blood serum oestradiol-17β and progesterone concentrations in goats during the luteal phase and the synchronized oestrus was investigated. Thirty-six Greek indigenous primiparous goats were used, during the oestrus period; 12 goats received, per os, 50 μg (treated group T50) and 12 goats received 100 μg (treated group T100) AFB/day/head, respectively, for approximately 1.5 month, while 12 goats served as controls (C). On day 36 of the experiment, each goat was injected, i.m, 0.5 ml prostaglandin F (PGF). Blood samples were collected from each goat twice a week, before PGF injection, as well as every 4 hours from the onset to the end of the synchronized oestrus. Oestradiol-17β and progesterone concentrations in blood serum were determined using radioimmunoassay. During the whole luteal(s) phase(s), linear regression analysis revealed a significant negative dependence (P < 0.05) of oestradiol-17β and a significant positive dependence (P < 0.05) of progesterone over group (C = 0, T50 = 50, T100 = 100), in a dose dependent manner. During the synchronized oestrus, multiple linear regression analysis revealed a significant negative dependence (P < 0.05) of oestradiol-17β, as well as a significant positive dependence (P < 0.05) of progesterone over group (C = 0, T50 = 50, T100 = 100) and over time (hours, from the onset to the end of the synchronized oestrus). No significant differences were noticed among the three groups, regarding the body weight of the goats from the onset to the end of AFB administration, the occurrence or the duration of the synchronized oestrus presented by the goats (P > 0.05). In conclusion, prolonged AFB administration at doses of 100 or even of 50 μg/day/head changes the hormonal pattern in blood during the luteal phase and the synchronized oestrus of goats, being in oestrus period.
研究了在黄体期和同期发情期间，长期给山羊投喂黄曲霉毒素B（AFB）对血清雌二醇-17β和孕酮浓度的影响。选用36只希腊本土初产山羊，在发情期进行实验；12只山羊每天每头经口投喂50μg AFB（处理组T50），12只山羊每天每头经口投喂100μg AFB（处理组T100），持续约1.5个月，另12只山羊作为对照（C组）。在实验的第36天，每只山羊肌肉注射0.5ml前列腺素F（PGF）。在注射PGF前，每周从每只山羊采集两次血样，在同期发情开始至结束期间，每4小时采集一次血样。采用放射免疫分析法测定血清中雌二醇-17β和孕酮的浓度。在整个黄体期，线性回归分析显示，雌二醇-17β呈显著负相关（P<0.05），孕酮呈显著正相关（P<0.05），且呈剂量依赖性（C组=0，T50组=50，T100组=100）。在同期发情期间，多元线性回归分析显示，雌二醇-17β呈显著负相关（P<0.05），孕酮呈显著正相关（P<0.05），且与组（C组=0，T50组=50，T100组=100）和时间（从同期发情开始至结束的小时数）有关。在AFB投喂开始至结束期间，三组山羊的体重、同期发情的发生情况或持续时间均无显著差异（P>0.05）。总之，在发情期，每天每头山羊长期投喂100μg甚至50μg的AFB会改变山羊黄体期和同期发情期间血液中的激素模式。
