Chen Mengdan, Cao Jinjun, Li Weihai, Wang Ying
Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang, China.
Mitochondrial DNA B Resour. 2019 Oct 26;4(2):3790-3791. doi: 10.1080/23802359.2019.1681918.
Claasseniini is the apparent sister group to all the other groups of Perlidae, and the small tribe Claasseniini has only one genus includes 12 species in the world. Only five mitochondrial genomes in the Perlinae have previously been reported. In this study, we sequenced the mitochondrial genome of sp. Wu in genus for the first time. The total length of the mitochondrial genome is 15,774 bp, and the DNA base composition is 34.0% A, 27.4% T, 25.6% C and 13.0% G. The whole genome contains 37 genes and one control region, which is similar to other published stoneflies. All PCG started with ATN except CCG for COI gene, TTG for ND1 gene, GTG for ND4 and ND5 genes. The termination codon of 11 PCGs used the normal complete codon TAA or TAG, and the COII and ND5 genes were terminated by a single T. All tRNAs except fold into a classic clover structure. The phylogenetic tree by Maximum likelihood (ML) and Bayesian (BI) methods supported that sp. was the basal taxa of the Perlidae clade. This study provides new data for further mitochondrial genome study on the Perlidae.
Claasseniini族是所有其他 Perlidae 类群的明显姐妹群，这个小部落 Claasseniini 在世界上只有一个属，包含12个物种。此前仅报道过 Perlinae 亚科中的五个线粒体基因组。在本研究中，我们首次对 属的 种 Wu 的线粒体基因组进行了测序。线粒体基因组全长15,774 bp，DNA碱基组成为34.0% 的A、27.4% 的T、25.6% 的C和13.0% 的G。整个基因组包含37个基因和一个控制区，这与其他已发表的石蝇相似。所有蛋白质编码基因（PCG）均以ATN起始，但COI基因以CCG起始，ND1基因以TTG起始，ND4和ND5基因以GTG起始。11个PCG的终止密码子使用正常的完整密码子TAA或TAG，COII和ND5基因以单个T终止。除 外的所有tRNA都折叠成经典的三叶草结构。通过最大似然法（ML）和贝叶斯法（BI）构建的系统发育树支持 种是 Perlidae 进化枝的基部类群。本研究为 Perlidae 的线粒体基因组进一步研究提供了新数据。
