Wang Ying, Cao Jinjun, Chen Jiajia, Li Weihai
Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang, China.
Mitochondrial DNA B Resour. 2020 Jul 14;5(3):2809-2810. doi: 10.1080/23802359.2020.1788465.
We sequenced the complete mitochondrial genomes of which is the unique species distributed in Japan to provide supplementary data for future study in phylogenetic studies of Perlidae. This complete mitogenome of is 15,915 bp long, including 37 typical genes and a control region as other stoneflies. Through the data analysis, the A + T content of the whole mitogenome, PCGs, tRNAs, rRNAs, and control region was 63.7%, 61.3%, 68.7%, 66.8%, and 74.8%. Most PCGs used typical start or stop codon except , , and used the exceptional start codon and the and used the single T as the stop codon. Phylogenetic tree was constructed by using Bayesian inference (BI) and maximum-likelihood (ML) analysis based on the sequences of the 13 PCGs and two rRNAs and the result showed that subfamily Perlinae was a monophyletic group and the clade and had a closer relationship.
我们对分布于日本的独特物种[物种名称未给出]的完整线粒体基因组进行了测序，以便为未来石蝇科系统发育研究提供补充数据。该[物种名称未给出]的完整线粒体基因组长度为15,915 bp，与其他石蝇一样，包含37个典型基因和一个控制区。通过数据分析，整个线粒体基因组、编码蛋白质的基因（PCGs）、转运RNA（tRNAs）、核糖体RNA（rRNAs）和控制区的A+T含量分别为63.7%、61.3%、68.7%、66.8%和74.8%。除了[基因名称未给出]、[基因名称未给出]和[基因名称未给出]使用特殊起始密码子，以及[基因名称未给出]和[基因名称未给出]使用单个T作为终止密码子外，大多数编码蛋白质的基因使用典型的起始或终止密码子。基于13个编码蛋白质的基因和两个核糖体RNA的序列，通过贝叶斯推断（BI）和最大似然（ML）分析构建了系统发育树，结果表明Perlinae亚科是一个单系类群，并且[类群名称未给出]和[类群名称未给出]分支关系更近。
