Liu Yinrong, Chen Wenna, Li Fang, Li Chan, Xie Xuena, Chao Zhi, Tian Enwei
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
College of Landscape and Ecological Engineering, Hebei University of Engineering, Handan, China.
Mitochondrial DNA B Resour. 2019 Nov 13;4(2):4041-4042. doi: 10.1080/23802359.2019.1689867.
The dry root (Radix Fici Hirtae) of has been used as a traditional herbal medicine in Ling nan regions of China for a long time. As its large market demand, the wild resources of have sharply reduced. It is necessary to conduct the study of conservation genetics. However, there is still lack of complete genome information for the research on evolutionary biology, population genetics and phylogeography of this species. Here, we sequenced the complete chloroplast (CP) genome of using Next Generation Sequencing technology (NGS). The CP genome of is 160,374 bp in length, which contains a large single-copy (LSC) region of 88,446 bp, a small sing-copy (SSC) region of 18,134 bp, and two inverted repeat (IRa and IRb) regions of 26,897 bp. A total of 130 genes were successfully annotated containing 85 protein-coding genes, 37 tRNA genes and 8 rRNA genes. Phylogenetic analysis support genus Ficus is monophyletic and is closely related to within this genus.
该植物的干燥根（五指毛桃根）在中国岭南地区长期被用作传统草药。由于其市场需求巨大，该植物的野生资源已急剧减少。因此有必要开展保护遗传学研究。然而，目前仍缺乏完整的基因组信息，无法用于该物种进化生物学、群体遗传学和系统地理学的研究。在此，我们利用新一代测序技术（NGS）对该植物的完整叶绿体（CP）基因组进行了测序。该植物的CP基因组长度为160,374 bp，其中包含一个88,446 bp的大单拷贝（LSC）区域、一个18,134 bp的小单拷贝（SSC）区域以及两个26,897 bp的反向重复（IRa和IRb）区域。共成功注释了130个基因，其中包括85个蛋白质编码基因、37个tRNA基因和8个rRNA基因。系统发育分析支持榕属为单系类群，且该植物在该属内与另一植物关系密切。
