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中国国家重点保护野生动物(食肉目:鼬科)的完整线粒体基因组。

Complete mitochondrial genome of (Carnivora: Mustelidae), a protected and endangered species in China.

作者信息

Gao Wei, Lu Zhihao, Liang Yukang, Ren Zhu-Mei

机构信息

School of Life Science, Shanxi University, Taiyuan, China.

College of Plant Protection, Nanjing Agricultural University, Nanjing, China.

出版信息

Mitochondrial DNA B Resour. 2020 Feb 7;5(1):1081-1083. doi: 10.1080/23802359.2020.1723447.

Abstract

We sequenced the complete mitochondrial genome (mitogenome) of in China by the shotgun genome skimming methods. The mitogenome of is 16,558bp in length with the base composition of 32.9% A, 27.3% T, 26.0% C, and 13.9% G, and consists of 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and one non-coding control region. The 13 PCGs use ATG as initiation codons except , and which initiate with codons ATA and ATT, respectively. Four (, , and ) of the 13 PCGs terminate with a single T- -, and the remainder with a TAA termination codon except and using TA- and AGA as termination codon. The phylogenetic tree based on 13 protein-coding genes indicated that is sister to the clade grouped with three species , , and , and Mustelinae species formed a monophyletic group, which is close to the Lutrinae clade within Mustelidae.

摘要

我们采用鸟枪法基因组扫描方法对中国境内的[物种名称]的完整线粒体基因组(线粒体基因组)进行了测序。[物种名称]的线粒体基因组长度为16,558bp,碱基组成为32.9%A、27.3%T、26.0%C和13.9%G,由13个蛋白质编码基因(PCGs)、22个tRNA、两个rRNA和一个非编码控制区组成。13个PCGs中,除了[基因名称1]、[基因名称2]和[基因名称3]分别以ATA和ATT作为起始密码子外,其余均以ATG作为起始密码子。13个PCGs中有4个([基因名称4]、[基因名称5]、[基因名称6]和[基因名称7])以单个T- -作为终止密码子,其余的以TAA作为终止密码子,但[基因名称8]和[基因名称9]分别使用TA-和AGA作为终止密码子。基于13个蛋白质编码基因构建的系统发育树表明,[物种名称]与由[物种名称1]、[物种名称2]、[物种名称3]三个物种组成的进化枝互为姐妹群,鼬亚科物种形成一个单系群,该单系群与鼬科内的水獭亚科进化枝关系较近。

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