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蔷薇科(Rosaceae)植物叶绿体全基因组的测序与特征分析

Sequencing and characterization of the complete chloroplast genome of (Rosaceae).

作者信息

Sun Wenguang, Chen Zhe, Lv Zhenyu, Li Zhimin

机构信息

School of Life Science, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Yunnan Normal University, Kunming, China.

Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.

出版信息

Mitochondrial DNA B Resour. 2020 Feb 6;5(1):1090-1091. doi: 10.1080/23802359.2020.1721359.

Abstract

is a species which is endemic to China. We utilized next-generation Illumina sequencing technology to sequence and assemble its chloroplast genome. The 160,137 bp genome contained four main sections, including a pair of inverted repeat regions which were 26,332 bp in length, and a small single-copy region was 19,418 bp, as well as a large single-copy region, which was 88,055 bp. The genome had a GC content of 36.1% and contained 113 unique genes, including 4 rRNAs, 30 tRNAs, and 79 protein-coding genes. The phylogenetic relationship of 25 Rosaceae species was constructed based on their chloroplast genome sequences, which supported a close relationship between and Amygdaloideae. This newly sequenced plastid genome provides useful information for assessing the genetic diversity and phylogenetic position of .

摘要

是中国特有的物种。我们利用新一代Illumina测序技术对其叶绿体基因组进行测序和组装。该160,137 bp的基因组包含四个主要部分,包括一对长度为26,332 bp的反向重复区域,一个19,418 bp的小单拷贝区域,以及一个88,055 bp的大单拷贝区域。该基因组的GC含量为36.1%,包含113个独特基因,包括4个rRNA、30个tRNA和79个蛋白质编码基因。基于25个蔷薇科物种的叶绿体基因组序列构建了系统发育关系,这支持了与扁桃亚科之间的密切关系。这个新测序的质体基因组为评估的遗传多样性和系统发育位置提供了有用信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8346/7748509/6d40987f842c/TMDN_A_1721359_F0001_B.jpg

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