Liang Yu-Kang, Wen Jun, Ren Zhu-Mei
School of Life Science, Shanxi University, Taiyuan, Shanxi, China.
Department of Botany, MRC-166, National Museum of Natural History, Smithsonian Institution, Washington, DC, USA.
Mitochondrial DNA B Resour. 2019 Jul 12;4(2):2363-2364. doi: 10.1080/23802359.2019.1629846.
We sequenced the complete mitochondrial genome of the Chinese gall aphid (Hemiptera: Aphididae: Eriosomatinae: Fordini) by the genome skimming method on an Illunima platform. The assembled mitogenome is 16,191 bp in length with a very high A + T content of 84.3%. This genome consists of 13 protein-coding genes, 22 tRNAs, 2 rRNAs, and a control region. All the protein-coding genes have a typical ATN initiation codon and TAA termination codon except COX1 and ND4 with a single T as stop codon. The tRNAs ranged in size from 59 to 77 bp and formed a clover-leaf secondary structure except tRNA-Ser (AGN). We constructed the phylogenetic relationship of Fordini aphids including all the gall aphids, and the ML tree showed that grouped with as its sister group.
我们通过Illunima平台上的基因组浅层测序方法，对中国五倍子蚜虫（半翅目：蚜科：绵蚜亚科：倍蚜族）的完整线粒体基因组进行了测序。组装后的线粒体基因组长度为16,191 bp，A+T含量非常高，达84.3%。该基因组由13个蛋白质编码基因、22个tRNA、2个rRNA和一个控制区组成。除COX1和ND4以单个T作为终止密码子外，所有蛋白质编码基因都具有典型的ATN起始密码子和TAA终止密码子。tRNA的大小在59至77 bp之间，除了tRNA-Ser（AGN）外，均形成了三叶草型二级结构。我们构建了包括所有五倍子蚜虫在内的倍蚜族蚜虫的系统发育关系，最大似然树显示， 与 聚在一起，互为姐妹群。
