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利用牛津纳米孔MinION测序仪获得的欧亚喜鹊五个亚种的完整线粒体基因组及其种内分类学解读。

Complete mitochondrial genomes of five subspecies of the Eurasian magpie , obtained with Oxford Nanopore MinION, and their interpretation regarding intraspecific taxonomy.

作者信息

Kryukov Alexey P, Spiridonova Liudmila N, Tyunin Alexey P, Kryukov Kirill A, Dorda Beatriz A

机构信息

Federal Scientific Center of the East Asia Terrestrial Biodiversity (FSCEATB), Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.

National Institute of Genetics, Mishima, Japan.

出版信息

Mitochondrial DNA B Resour. 2020 Nov 20;5(3):3810-3811. doi: 10.1080/23802359.2020.1838354.

Abstract

The complete mitochondrial (mt) genomes of five subspecies of the Eurasian (Common) magpie were determined for the first time. Lengths of the circular genomes comprise 13 protein-coding genes, two rRNA genes (for 12S rRNA and 16S rRNA), 22 tRNA genes, and the non-coding control region (CR). Gene content and lengths of the genomes (16,936-16,945 bp) are similar to typical vertebrate mt genomes. The subspecies studied differs by several single substitutions and indels, especially in the CR. The phylogenetic tree based on complete mt genomes shows a deep divergence of the two groups of subspecies which supports the proposed division into two distinct species: and .

摘要

首次测定了欧亚喜鹊五个亚种的完整线粒体(mt)基因组。环状基因组的长度包括13个蛋白质编码基因、两个rRNA基因(12S rRNA和16S rRNA)、22个tRNA基因以及非编码控制区(CR)。基因组的基因组成和长度(16,936 - 16,945 bp)与典型的脊椎动物线粒体基因组相似。所研究的亚种存在若干单碱基替换和插入缺失差异,尤其是在控制区。基于完整线粒体基因组构建的系统发育树显示,两组亚种之间存在深度分化,这支持了将其划分为两个不同物种的提议: 和 。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b9a/7759274/1447b26e0d6a/TMDN_A_1840937_F0001_C.jpg

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