Zeng Liugen, Wang Junhua, Sheng Junqing, Gu Qing, Hong Yijiang
Key Laboratory of Molecular Biology and Genetic Engineering in Jiangxi, College of Life Science and Food Engineering, Nanchang University, Nanchang, PR China.
Mitochondrial DNA. 2012 Jun;23(3):187-200. doi: 10.3109/19401736.2012.668893.
The goal of our study was to investigate the molecular characteristics of mitochondrial DNA (mtDNA) and phylogenetic construction of the weather loach (Misgurnus anguillicaudatus) in Poyang Lake. The complete mitochondrial genome was 16,634 bp, and the gene order was identical to that of teleost fishes. Compared with the previous reported weather loach in China, there were numerous nucleotide substitutions and length polymorphisms on the structural genes of mitochondrial DNA in the loach from the Poyang Lake. The Phylogenetic tree indicated that the loach had its own molecular characteristics and was somewhat different from those in other regions of China. Fourteen unique haplotypes of the cytochrome b (cyt b) gene were obtained from 300 weather loaches. The Phylogenetic tree based on the cyt b gene showed that the loaches were substructured into two different populations in The Poyang Lake. Results indicated that the loaches in Poyang Lake not only showed the same phylogeny as the loaches in other areas of China, but also generated its own unique phylogenetic relationships.
我们研究的目的是调查鄱阳湖泥鳅（Misgurnus anguillicaudatus）线粒体DNA（mtDNA）的分子特征并构建其系统发育树。线粒体基因组全长16,634 bp，基因排列顺序与硬骨鱼类相同。与之前报道的中国泥鳅相比，鄱阳湖泥鳅线粒体DNA结构基因上存在大量核苷酸替换和长度多态性。系统发育树表明，该泥鳅具有自身的分子特征，与中国其他地区的泥鳅有所不同。从300尾泥鳅中获得了细胞色素b（cyt b）基因的14种独特单倍型。基于cyt b基因构建的系统发育树显示，鄱阳湖的泥鳅分为两个不同的种群。结果表明，鄱阳湖的泥鳅不仅与中国其他地区的泥鳅具有相同的系统发育关系，还产生了自身独特的系统发育关系。
