Luckins A G
Trop Anim Health Prod. 1977 Feb;9(1):53-62. doi: 10.1007/BF02297393.
A micromodification of the enzyme-linked immunosorbent assay (ELISA) was evaluated for its potential application in the immunodiagonsis of bovine trypanosomiasis. Serum samples from infected and non-infected Zebu cattle and samples from Friesian cattle with experimental infections were exmained for the presence of trypanosomal antibodies. There were significant differences between the microELISA values obtained with samples from infected and non-infected cattle. During the course of infection microELISA values were found to fluctuate and the antibody response varied in individual animals. The test did not distinguish between infections with Trypanosoma brucei, T. vivax and T. congolense. There were no cross-reactions between trypanosome antigens and serum samples from cattle infected with T. theileri, Theileria parva, Th. mutans, Th. annulata, Babesia divergens and Anaplasma marginale.
对酶联免疫吸附测定（ELISA）的一种微量改良方法进行了评估，以确定其在牛锥虫病免疫诊断中的潜在应用。检测了来自感染和未感染的瘤牛血清样本以及来自实验感染的黑白花牛的样本中是否存在锥虫抗体。感染牛和未感染牛的样本所获得的微量ELISA值存在显著差异。在感染过程中，发现微量ELISA值会波动，且个体动物的抗体反应有所不同。该检测方法无法区分布氏锥虫、活泼锥虫和刚果锥虫感染。锥虫抗原与感染泰勒虫、小泰勒虫、突变泰勒虫、环形泰勒虫、分歧巴贝斯虫和边缘无浆体的牛的血清样本之间没有交叉反应。
