Chair of Bioprocess Engineering, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany.
ANiMOX GmbH, Berlin, Germany.
Biotechnol Lett. 2021 Mar;43(3):579-587. doi: 10.1007/s10529-020-03065-y. Epub 2020 Dec 26.
The rapid accumulation of crude-oil based plastics in the environment is posing a fundamental threat to the future of mankind. The biodegradable and bio-based polyhydroxyalkanoates (PHAs) can replace conventional plastics, however, their current production costs are not competitive and therefore prohibiting PHAs from fulfilling their potential.
Different low-quality animal by-products, which were separated by thermal hydrolysis into a fat-, fat/protein-emulsion- and mineral-fat-mixture- (material with high ash content) phase, were successfully screened as carbon sources for the production of PHA. Thereby, Ralstonia eutropha Re2058/pCB113 accumulated the short- and medium-chain-length copolymer poly(hydroxybutyrate-co-hydroxyhexanoate) [P(HB-co-HHx)]. Up to 90 wt% PHA per cell dry weight with HHx-contents of 12-26 mol% were produced in shake flask cultivations.
In future, the PHA production cost could be lowered by using the described animal by-product streams as feedstock.
原油基塑料在环境中的快速积累对人类的未来构成了根本性的威胁。可生物降解和生物基聚羟基烷酸酯 (PHA) 可以替代传统塑料，然而，它们目前的生产成本没有竞争力，因此限制了 PHA 发挥其潜力。
不同低质量的动物副产品，通过热水解分为脂肪、脂肪/蛋白质乳液和矿物质脂肪混合物（高灰分含量的物质）相，被成功筛选为生产 PHA 的碳源。由此，恶臭假单胞菌 Re2058/pCB113 积累了短链和中链长度共聚物聚（羟基丁酸酯-co-羟基己酸酯）[P(HB-co-HHx)]。在摇瓶培养中，每细胞干重可生产高达 90wt%的 PHA，HHx 含量为 12-26mol%。
未来，可以使用所描述的动物副产品流作为原料来降低 PHA 的生产成本。
