从头确定肽结构的微 ED 法。
Ab Initio Determination of Peptide Structures by MicroED.
机构信息
Department of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, STROBE, NSF Science and Technology Center, University of California, Los Angeles (UCLA), Los Angeles, CA, USA.
Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry & Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, CA, USA.
出版信息
Methods Mol Biol. 2021;2215:329-348. doi: 10.1007/978-1-0716-0966-8_17.
Structural elucidation of small macromolecules such as peptides has recently been facilitated by a growing number of technological advances to existing crystallographic methods. The emergence of electron micro-diffraction (MicroED) of protein nanocrystals under cryogenic conditions has enabled the interrogation of crystalline peptide assemblies only hundreds of nanometers thick. Collection of atomic or near-atomic resolution data by these methods has permitted the ab initio determination of structures of various amyloid-forming peptides, including segments derived from prions and ice-nucleating proteins. This chapter focuses on the process of ab initio structural determination from nano-scale peptide assemblies and other similar molecules.
近年来，随着现有晶体学方法的技术进步，对小的大分子（如肽）的结构解析变得更加容易。在低温条件下对蛋白质纳米晶体进行电子微衍射（MicroED）的出现，使得人们能够研究只有几百纳米厚的结晶肽组装体。这些方法可以收集原子或近原子分辨率的数据，从而可以从头确定各种形成淀粉样蛋白的肽的结构，包括朊病毒和冰核蛋白衍生的片段。本章重点介绍了从头确定纳米级肽组装体和其他类似分子结构的过程。
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