Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.
Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
J Cell Biochem. 2021 Apr;122(3-4):315-325. doi: 10.1002/jcb.29714. Epub 2020 Dec 27.
It has been demonstrated in previous studies that lncPART1 is dysregulated in non-small cell lung cancer (NSCLC). However, the function of lncPART1 in NSCLC is unclear. Therefore, this experimental design was based on LncPART1 to explore the pathogenesis of NSCLC. Real-time polymerase chain reaction was used to detect the expression of lncPART1 and miR-17-5p in NSCLC. Cell Counting Kit -8, colony formation, and transwell assays were used to examine the effects of lncPART1 and miR-17-5p on NSCLC cell proliferation and migration invasiveness. Target gene prediction, luciferase reporter assays were used to validate downstream target genes for lncPART1 and miR-17-5p. Western blot analysis was used to detect the expression of TGFBETAR2. LncPART1 was highly expressed in NSCLC. LncPART1 significantly promoted cell proliferation of NSCLC cells. miR-17-5p was down-expressed in NSCLC. miR-17-5p overexpression inhibited cell proliferation and migration invasion in NSCLC cells. LncPART1 was able to inhibit miR-17-5p expression and upregulate the expression level of TGFBETAR2. The results of in vivo animal models confirmed that lncPART1 promoted NSCLC progression by miR-17-5p/TGFBETAR2 axis. LncPART1 promoted the progression of NSCLC by miR-17-5p/TGFBETAR2 axis.
先前的研究表明，lncPART1 在非小细胞肺癌（NSCLC）中失调。然而，lncPART1 在 NSCLC 中的功能尚不清楚。因此，本实验设计基于 LncPART1 来探讨 NSCLC 的发病机制。实时聚合酶链反应用于检测 NSCLC 中 lncPART1 和 miR-17-5p 的表达。细胞计数试剂盒-8、集落形成和 Transwell 分析用于检测 lncPART1 和 miR-17-5p 对 NSCLC 细胞增殖和迁移侵袭的影响。靶基因预测、荧光素酶报告实验用于验证 lncPART1 和 miR-17-5p 的下游靶基因。Western blot 分析用于检测 TGFBETAR2 的表达。lncPART1 在 NSCLC 中高表达。lncPART1 显著促进 NSCLC 细胞的增殖。miR-17-5p 在 NSCLC 中表达下调。miR-17-5p 过表达抑制 NSCLC 细胞的增殖和迁移侵袭。lncPART1 能够抑制 miR-17-5p 的表达并上调 TGFBETAR2 的表达水平。体内动物模型的结果证实，lncPART1 通过 miR-17-5p/TGFBETAR2 轴促进 NSCLC 的进展。lncPART1 通过 miR-17-5p/TGFBETAR2 轴促进 NSCLC 的进展。
