Buckley G, Benson M T, Jenkinson E J, Owen J J
Department of Anatomy, University of Birmingham Medical School, England.
Transplantation. 1988 Jan;45(1):202-5. doi: 10.1097/00007890-198801000-00041.
dGuo-treated mouse embryo thymus lobes depleted of lymphoid and dendritic cells are not rejected by normal allogeneic mice despite expression of class I and class II major histocompatibility (MHC) antigens on thymic epithelial cells. Our results suggest that host mice are not tolerant to donor antigens, but treated lobes survive because thymic epithelial cells are not immunogenic. Multiple grafts of dGuo-treated lobes are rejected perhaps because numbers of residual dendritic cells reach a threshold necessary for priming. Mice previously primed by injection of spleen cells reject dGuo-treated thymus lobes, and crossreactions between major and minor histocompatibility antigens can be demonstrated. The acceptance of dGuo-treated thymus allografts by normal mice provides a system for investigating the requirements for priming.
经二氢叶酸还原酶抑制剂（dGuo）处理的小鼠胚胎胸腺叶，其淋巴细胞和树突状细胞已被清除，尽管胸腺上皮细胞上表达了Ⅰ类和Ⅱ类主要组织相容性（MHC）抗原，但仍不会被正常同种异体小鼠排斥。我们的结果表明，宿主小鼠并非对供体抗原产生耐受，而是经处理的胸腺叶能够存活，因为胸腺上皮细胞没有免疫原性。多次移植经dGuo处理的胸腺叶可能会被排斥，原因或许是残余树突状细胞的数量达到了启动免疫反应所需的阈值。先前通过注射脾细胞进行致敏的小鼠会排斥经dGuo处理的胸腺叶，并且可以证明主要和次要组织相容性抗原之间存在交叉反应。正常小鼠对经dGuo处理的胸腺同种异体移植物的接受为研究启动免疫反应的条件提供了一个系统。
