Ready A R, Jenkinson E J
Department of Anatomy, University of Birmingham, Edgbaston, UK.
Immunology. 1994 Nov;83(3):465-8.
Deoxyguanosine (dGuo)-treated fetal thymus lobes are capable of prolonged survival in histoincompatible recipients despite their expression of both class I and class II major histocompatibility complex (MHC) antigens. Although dGuo treatment has been directly shown to eliminate lymphocytes from the lobes its effect upon other marrow-derived passenger cells such as macrophages and dendritic cells is less well defined. Here we show that dGuo-treated CBA(H-2k) fetal thymus lobes allowed to develop under the renal capsule of immunoincompetent BALB/c (H-2d) mice for 3 weeks are depleted of donor-type dendritic cells in contrast to grafts of untreated lobes where donor-derived dendritic cells are still detectable at this time. Moreover, dGuo-treated thymus lobes underwent prompt allo-rejection if recolonized with donor-type dendritic cells prior to transplantation into immunocompetent recipients. Together with our observation that macrophages (or their precursors) survive dGuo treatment, these results suggest that the reduced immunogenicity of fetal thymus grafts seen following dGuo treatment is related to dendritic cell, rather than macrophage depletion.
脱氧鸟苷（dGuo）处理过的胎儿胸腺叶尽管同时表达I类和II类主要组织相容性复合体（MHC）抗原，但仍能够在组织不相容的受体中长期存活。虽然已直接证明dGuo处理可从胸腺叶中清除淋巴细胞，但其对其他骨髓来源的过客细胞（如巨噬细胞和树突状细胞）的影响尚不明确。在此我们表明，在免疫缺陷的BALB/c（H-2d）小鼠肾被膜下培养3周的dGuo处理的CBA（H-2k）胎儿胸腺叶，与未处理的胸腺叶移植相比，此时未处理的胸腺叶中仍可检测到供体来源的树突状细胞，而经dGuo处理的胸腺叶中供体型树突状细胞已被耗尽。此外，如果在移植到有免疫活性的受体之前用供体型树突状细胞重新定植，经dGuo处理的胸腺叶会迅速发生同种异体排斥反应。结合我们观察到巨噬细胞（或其前体）在dGuo处理后存活的结果，这些结果表明，dGuo处理后胎儿胸腺移植物免疫原性降低与树突状细胞而非巨噬细胞的耗竭有关。
