Koo S I, Lee C C
Department of Biochemistry, Oral Roberts University School of Medicine, Tulsa, OK 74171.
Am J Clin Nutr. 1988 Jan;47(1):120-7. doi: 10.1093/ajcn/47.1.120.
This study investigated the effect of zinc status on the lipid and apolipoprotein composition of high-density lipoproteins (HDL) in adult male rats fed a semipurified diet containing a marginal level (2.8 mg/kg) of Zn as compared with the pair-fed and ad libitum-fed controls given an adequate level (30.8 mg/kg) of Zn for 7-10 wk. Compositional and chromatographic analysis of HDL fraction showed that Zn deficiency significantly lowered the total amount of circulating HDL particles with no effect on the concentrations of total protein, triglyceride, phospholipid, and cholesterol. The ratios of free: esterified cholesterol remained the same for the three groups. Electrophoretic analysis of the apolipoprotein profile showed marked decreases in the relative contents of apolipoproteins E and C and an increase in apolipoprotein (apo) A-I with no significant change in apo A-VI. No apparent morphologic aberrations of HDL particles were observed. Such changes may significantly influence the transport and metabolism of cholesterol and may provide an explanation for altered cholesterol homeostasis in Zn deficiency.
本研究调查了锌状态对成年雄性大鼠高密度脂蛋白（HDL）脂质和载脂蛋白组成的影响。给成年雄性大鼠喂食含边缘水平（2.8毫克/千克）锌的半纯化日粮，同时设置配对喂食和自由采食的对照组，给予充足水平（30.8毫克/千克）的锌，持续7至10周。HDL组分的成分和色谱分析表明，锌缺乏显著降低了循环HDL颗粒的总量，而对总蛋白、甘油三酯、磷脂和胆固醇的浓度没有影响。三组的游离胆固醇与酯化胆固醇的比例保持不变。载脂蛋白谱的电泳分析显示，载脂蛋白E和C的相对含量显著降低，载脂蛋白（apo）A-I增加，而apo A-VI没有显著变化。未观察到HDL颗粒明显的形态异常。这些变化可能会显著影响胆固醇的转运和代谢，并可能为锌缺乏时胆固醇稳态改变提供解释。
