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源自人类急性髓系白血病细胞系的诱导多能干细胞系（HL-60-iPSC）保留白血病异常特征并表现出髓系分化缺陷。

An iPSC line derived from a human acute myeloid leukemia cell line (HL-60-iPSC) retains leukemic abnormalities and displays myeloid differentiation defects.

作者信息

Yamasaki Amanda E, Warshaw Jane N, Kyalwazi Beverly L, Matsui Hiroko, Jepsen Kristen, Panopoulos Athanasia D

机构信息

Department of Biological Sciences, University of Notre Dame, IN 46556, USA; Center for Stem Cells and Regenerative Medicine, University of Notre Dame, IN 46556, USA.

Department of Biological Sciences, University of Notre Dame, IN 46556, USA.

出版信息

Stem Cell Res. 2020 Dec;49:102096. doi: 10.1016/j.scr.2020.102096. Epub 2020 Nov 23.

DOI:10.1016/j.scr.2020.102096
PMID:33370871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8031422/
Abstract

Cancer-derived iPSCs have provided valuable insight into oncogenesis, but human cancer cells can often be difficult to reprogram, especially in cases of complex genetic abnormalities. Here we report, to our knowledge, the first successful generation of an iPSC line from a human immortalized acute myeloid leukemia (AML) cell line, the cell line HL-60. This iPSC line retains a majority of the leukemic genotype and displays defects in myeloid differentiation, thus providing a tool for modeling and studying AML.

摘要

癌症衍生的诱导多能干细胞为肿瘤发生提供了有价值的见解，但人类癌细胞往往难以重编程，尤其是在复杂基因异常的情况下。据我们所知，在此我们报告首次成功从人类永生化急性髓系白血病（AML）细胞系HL-60生成了诱导多能干细胞系。该诱导多能干细胞系保留了大部分白血病基因型，并在髓系分化方面存在缺陷，从而为AML的建模和研究提供了一个工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8499/8031422/8b96a388ec86/nihms-1658120-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8499/8031422/8b96a388ec86/nihms-1658120-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8499/8031422/8b96a388ec86/nihms-1658120-f0001.jpg

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本文引用的文献

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High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells.高通量且经济高效的诱导多能干细胞表征。
Stem Cell Reports. 2017 Apr 11;8(4):1101-1111. doi: 10.1016/j.stemcr.2017.03.011.
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iPSCORE: A Resource of 222 iPSC Lines Enabling Functional Characterization of Genetic Variation across a Variety of Cell Types.iPSCORE：一个包含 222 个人诱导多能干细胞系的资源库，能够对多种细胞类型中的遗传变异进行功能特征分析。
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急性髓系白血病患者细胞的重编程：携带癌症突变需要靶向 AML 层级。
Stem Cells Transl Med. 2023 Jun 15;12(6):334-354. doi: 10.1093/stcltm/szad022.
4
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HL-60 cell line was derived from a patient with FAB-M2 and not FAB-M3.HL-60细胞系源自一名患有FAB-M2而非FAB-M3的患者。
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Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia.对一名急性早幼粒细胞白血病患者的连续分化髓系细胞系（HL-60）的特征描述。
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