The Smart Materials Research Institute, Southern Federal University, Sladkova Street 178/24, 344090 Rostov-on-Don, Russia.
Research Laboratory "Biomedicine", Southern Federal University, Stachki av. 194/1, 344090 Rostov-on-Don, Russia.
Int J Mol Sci. 2020 Dec 21;21(24):9758. doi: 10.3390/ijms21249758.
Synthesis of the MIL-100 metal-organic framework particles was carried out by hydrothermal (HT) and microwave (MW)-assisted methods. Transmission electron microscopy showed formation of microparticles in the course of hydrothermal synthesis and nanoparticles for microwave-assisted synthesis. Powder X-ray diffraction confirmed formation of larger crystallites for hydrothermal synthesis. Particle aggregation in aqueous solution was observed by dynamic light scattering. However, the stability of both samples could be improved in acetic acid solution. Nitrogen sorption isotherms showed high porosity of the particles. ᶫ-leucine molecule was used as a model molecule for loading in the porous micro- and nanoparticles. Loading was estimated by FTIR spectroscopy and thermogravimetric analysis. UV-VIS spectroscopy quantified ᶫ-leucine release from the particles in aqueous solution. Cytotoxicity studies using the HeLa cell model showed that the original particles were somewhat toxic, but ᶫ-leucine loading ameliorated the toxic effects, likely due to signaling properties of the amino acid.
MIL-100 金属有机骨架颗粒的合成采用水热（HT）和微波（MW）辅助方法进行。透射电子显微镜显示，在水热合成过程中形成了微颗粒，而在微波辅助合成中形成了纳米颗粒。粉末 X 射线衍射证实了水热合成形成了较大的晶体。通过动态光散射观察到颗粒在水溶液中的聚集。然而，在乙酸溶液中可以提高两种样品的稳定性。氮气吸附等温线表明颗粒具有高孔隙率。β-亮氨酸分子被用作模型分子，用于负载在多孔微球和纳米颗粒中。通过傅里叶变换红外光谱和热重分析估算了负载量。紫外-可见光谱定量测定了β-亮氨酸从颗粒在水溶液中的释放。使用 HeLa 细胞模型的细胞毒性研究表明，原始颗粒具有一定的毒性，但β-亮氨酸负载改善了毒性作用，可能是由于氨基酸的信号特性。
