Lorusso Felice, Inchingolo Francesco, Dipalma Gianna, Postiglione Francesca, Fulle Stefania, Scarano Antonio
Department of Innovative Technologies in Medicine & Dentistry, University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
Department of Interdisciplinary Medicine, University of Bari "Aldo Moro", 70121 Bari, Italy.
Int J Mol Sci. 2020 Dec 21;21(24):9765. doi: 10.3390/ijms21249765.
Recently a greater interest in tissue engineering for the treatment of large bone defect has been reported. The aim of the present systematic review and meta-analysis was to investigate the effectiveness of dental pulp stem cells and synthetic block complexes for bone defect treatment in preclinical in vivo articles.
The electronic database and manual search was conducted on Pubmed, Scopus, and EMBASE. The papers identified were submitted for risk-of-bias assessment and classified according to new bone formation, bone graft characteristics, dental pulp stem cells (DPSCs) culture passages and amount of experimental data. The meta-analysis assessment was conducted to assess new bone formation in test sites with DPSCs/synthetic blocks vs. synthetic block alone.
The database search identified a total of 348 papers. After the initial screening, 30 studies were included, according to the different animal models: 19 papers on rats, 3 articles on rabbits, 2 manuscripts on sheep and 4 papers on swine. The meta-analysis evaluation showed a significantly increase in new bone formation in favor of DPSCs/synthetic scaffold complexes, if compared to the control at 4 weeks (Mean Diff: 17.09%, 95% CI: 15.16-18.91%, < 0.01) and at 8 weeks (Mean Diff: 14.86%, 95% CI: 1.82-27.91%, < 0.01) in rats calvaria bone defects.
The synthetic scaffolds in association of DPSCs used for the treatment of bone defects showed encouraging results of early new bone formation in preclinical animal studies and could represent a useful resource for regenerative bone augmentation procedures.
最近有报道称，人们对用于治疗大骨缺损的组织工程学兴趣日增。本系统评价和荟萃分析的目的是，在临床前体内研究文章中，调查牙髓干细胞和合成块状复合物治疗骨缺损的有效性。
在PubMed、Scopus和EMBASE上进行电子数据库检索和手工检索。对识别出的论文进行偏倚风险评估，并根据新骨形成、骨移植特征、牙髓干细胞（DPSCs）培养传代和实验数据量进行分类。进行荟萃分析评估，以评估在测试部位使用DPSCs/合成块与单独使用合成块相比的新骨形成情况。
数据库检索共识别出348篇论文。初步筛选后，根据不同动物模型纳入了30项研究：19篇关于大鼠的论文、3篇关于兔子的文章、2篇关于绵羊的手稿和4篇关于猪的论文。荟萃分析评估显示，与对照组相比，在大鼠颅骨骨缺损中，4周时（平均差异：17.09%，95%置信区间：15.16 - 18.91%，P < 0.01）和8周时（平均差异：14.86%，95%置信区间：1.82 - 27.91%，P < 0.01），使用DPSCs/合成支架复合物的新骨形成显著增加。
在临床前动物研究中，用于治疗骨缺损的与DPSCs联合的合成支架显示出早期新骨形成的令人鼓舞的结果，并且可能代表再生骨增量手术的一种有用资源。
