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环介导等温扩增技术(LAMP):一种用于肉类产品质量评估的快速灵敏工具。

Loop-Mediated Isothermal Amplification (LAMP): A Rapid and Sensitive Tool for Quality Assessment of Meat Products.

作者信息

Kumar Yogesh, Bansal Sangita, Jaiswal Pranita

机构信息

ICAR-Central Inst. of Post-Harvest Engineering and Technology (CIPHET), Ludhiana, India.

出版信息

Compr Rev Food Sci Food Saf. 2017 Nov;16(6):1359-1378. doi: 10.1111/1541-4337.12309. Epub 2017 Oct 12.

Abstract

Loop-mediated isothermal amplification (LAMP) is a novel method that amplifies target nucleic acids under isothermal conditions. It is a rapid, specific, and sensitive method, which does not require costly thermal cyclers for the detection of nucleic acids. Thus, it is suitable for on-site detection assays under low-resource settings. It can also be integrated on compact lab-on-a-chip devices for the development of micro-total analysis systems. This review discusses LAMP-based methods, as well as LAMP-based centrifugal, microfluidic, and other fluid-handling devices, which have been developed for the assessment of meat quality parameters that are related to the presence or absence of nucleic acids, for example, animal species identification and microbiological quality. Advances in improving the rapidity, specificity, and sensitivity of LAMP techniques for the assessment of these meat quality parameters are also discussed in this review.

摘要

环介导等温扩增(LAMP)是一种在等温条件下扩增靶核酸的新方法。它是一种快速、特异且灵敏的方法,检测核酸时无需昂贵的热循环仪。因此,它适用于资源匮乏环境下的现场检测分析。它还可集成到紧凑型芯片实验室设备上,用于开发微全分析系统。本综述讨论了基于LAMP的方法以及基于LAMP的离心、微流控和其他流体处理设备,这些设备已被开发用于评估与核酸存在与否相关的肉质参数,例如动物物种鉴定和微生物质量。本综述还讨论了在提高LAMP技术评估这些肉质参数的速度、特异性和灵敏度方面取得的进展。

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