Ling Hangjian, Sridhar Kaushik, Gollapudi Sumanth, Kumar Jyoti, Ohgami Robert S
Department of Mechanical Engineering, University of Massachusetts Dartmouth, 285 Old Westport Road, Dartmouth, MA 02747, USA.
Department of Pathology, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA, and.
Microscopy (Oxf). 2021 Aug 9;70(4):333-339. doi: 10.1093/jmicro/dfaa077.
The measurement of the volume of blood cells is important for clinical diagnosis and patient management. While digital holography microscopy has been used to obtain such information, previous off-axis setups usually involve a separated reference beam and are thus not very easy to implement. Here, we use the simple in-line Gabor setup without separation of a reference beam to measure the shape and volume of cells mounted on glass slides. Inherent to the in-line holograms, the reconstructed phase of the object is affected by the virtual image noise, producing errors in the cell volume measurement. We optimized our approach to use a single hologram without phase retrieval, increasing distance between cell and hologram plane to reduce the measurement error of cell volume to less than 6% in some instances. Therefore, the in-line Gabor setup can be a useful and simple tool to obtain volumetric and morphologic cellular information.
血细胞体积的测量对于临床诊断和患者管理至关重要。虽然数字全息显微镜已被用于获取此类信息，但以前的离轴设置通常涉及分离的参考光束，因此不太容易实现。在这里，我们使用简单的同轴加博尔设置，无需分离参考光束来测量安装在载玻片上的细胞的形状和体积。由于同轴全息图的固有特性，物体的重建相位会受到虚像噪声的影响，从而在细胞体积测量中产生误差。我们优化了方法，使用单个全息图而无需相位检索，增加细胞与全息图平面之间的距离，在某些情况下将细胞体积的测量误差降低到小于6%。因此，同轴加博尔设置可以成为获取细胞体积和形态信息的有用且简单的工具。
