Department of Laboratory Medicine, Tenri Hospital, Tenri City, Nara, Japan.
Department of Clinical Laboratory Science, Tenri Health Care University, Tenri City, Nara, Japan.
Clin Appl Thromb Hemost. 2020 Jan-Dec;26:1076029620978810. doi: 10.1177/1076029620978810.
Clot waveform analysis based on activated partial thromboplastin time (aPTT) is reported to be a useful assay. We attempted to find beneficial parameters with the first-derivative curve. We examined 106 plasma samples with prolonged aPTT and analyzed the first-derivative curve statistically by dividing it into 6 groups (Lupus anticoagulant, Heparin, Direct oral anticoagulants, Factor VIII inhibitor, Hepatic dysfunctions and Factor deficiency). We obtained 7 coordinates for parameter measurement by analyzing the first-derivative curve and set 20 parameters including the velocity axis, the time axis, and area parameters. The distribution was checked by extracting each parameter that showed the most significant difference in the 6 groups. As a result, it was revealed that we could classify aPTT prolongation by using a combination of 3 parameters, the initial-to-peak gradient, the ratio initial-to-intermediate velocity/intermediate-to-peak velocity, and the initial-to-peak area size. We constructed a flowchart combining these 3 parameters and were able to discriminate 75% of the specimens. These parameters derived from the first-derivative curve of clot waveform analysis are useful tools to discriminate aPTT prolongation.
基于活化部分凝血活酶时间 (aPTT) 的血栓波形分析据报道是一种有用的检测方法。我们试图通过求导曲线找到有益的参数。我们检查了 106 份 aPTT 延长的血浆样本，并通过将其分为 6 组（狼疮抗凝物、肝素、直接口服抗凝剂、VIII 因子抑制剂、肝功能障碍和因子缺乏）对其求导曲线进行了统计学分析。我们通过分析求导曲线获得了 7 个用于参数测量的坐标，并设置了 20 个参数，包括速度轴、时间轴和面积参数。通过提取 6 组中差异最显著的每个参数来检查分布情况。结果表明，我们可以使用 3 个参数的组合，即初始至峰值梯度、初始至中间速度/中间至峰值速度的比值以及初始至峰值面积大小来对 aPTT 延长进行分类。我们结合这 3 个参数构建了一个流程图，可以区分 75%的样本。这些源自血栓波形分析求导曲线的参数是区分 aPTT 延长的有用工具。
