Macromolecular Chemistry, Institute of Chemistry, Faculty of Natural Science II (Chemistry, Physics and Mathematics), Martin Luther University Halle-Wittenberg, von-Danckelmann-Platz 4, 06120, Halle, Germany.
Physical Chemistry, Institute of Chemistry, Faculty of Natural Science II (Chemistry, Physics and Mathematics), Martin Luther University Halle-Wittenberg, von-Danckelmann-Platz 4, 06120, Halle, Germany.
Angew Chem Int Ed Engl. 2021 Mar 29;60(14):7820-7827. doi: 10.1002/anie.202015179. Epub 2021 Feb 25.
Single-chain nanoparticles (SCNPs) are highly versatile structures resembling proteins, able to function as catalysts or biomedical delivery systems. Based on their synthesis by single-chain collapse into nanoparticular systems, their internal structure is complex, resulting in nanosized domains preformed during the crosslinking process. In this study we present proof of such nanocompartments within SCNPs via a combination of electron paramagnetic resonance (EPR) and fluorescence spectroscopy. A novel strategy to encapsulate labels within these water dispersible SCNPs with hydrodynamic radii of ≈5 nm is presented, based on amphiphilic polymers with additional covalently bound labels, attached via the copper catalyzed azide/alkyne "click" reaction (CuAAC). A detailed profile of the interior of the SCNPs and the labels' microenvironment was obtained via electron paramagnetic resonance (EPR) experiments, followed by an assessment of their photophysical properties.
单链纳米颗粒（SCNPs）是高度通用的结构，类似于蛋白质，能够作为催化剂或生物医学输送系统发挥作用。基于它们通过单链折叠成纳米颗粒系统的合成，其内部结构复杂，导致交联过程中预先形成纳米尺寸的域。在这项研究中，我们通过电子顺磁共振（EPR）和荧光光谱学的组合证明了 SCNPs 内存在这种纳米隔室。提出了一种在这些水可分散 SCNPs 中封装标记物的新策略，这些 SCNPs 的水动力半径约为 5nm，基于具有额外共价结合标记物的两亲聚合物，通过铜催化的叠氮化物/炔烃“点击”反应（CuAAC）连接。通过电子顺磁共振（EPR）实验获得了 SCNPs 内部和标记物微环境的详细概况，然后评估了它们的光物理性质。
